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Oligo Synthesis

Oligo Synthesis : Click Chemistry

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1-Ethynyl-dSpacer CE Phosphoramidite

1-Ethynyl-dSpacer CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1910-021-Ethynyl-dSpacer CE Phosphoramidite0.25g £1,355.00 Quantity Add to Order
10-1910-901-Ethynyl-dSpacer CE Phosphoramidite100umole £355.00 Quantity Add to Order
10-1910-951-Ethynyl-dSpacer CE Phosphoramidite50umole £191.00 Quantity Add to Order

Description

1-Ethynyl-dSpacer CE Phosphoramidite

5'-O-Dimethoxytrityl-1'-ethynyl-2'-deoxyribose-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C37H45N2O6P
M.W.: 644.74
F.W.: 204.12

1-Ethynyl-dSpacer CE Phosphoramidite can be used in any position within an oligonucleotide while still retaining the high efficiency of click chemistry. The modifier is efficiently incorporated into oligonucleotides using standard phosphoramidite chemistry, is stable to common deprotection conditions, and is compatible with Glen-Pak™ purification. 1-Ethynyl-dSpacer generates a substituted 1,2,3-triazole pseudo-nucleobase after click chemistry conjugation with an azide The 1-ethynyl-dSpacer modification exhibits similar duplex stability to the standard dSpacer (10-1914) and destabilizes the duplex when internally incorporated. Upon cycloaddition, the duplex stability is moderated by the resulting structure of the modification. Simple 1,2,3-triazoles were destabilizing, as were modifications that incorporated TEG linkers (6-FAM-TEG and Amino-TEG). Modifications that incorporated aromatic functional groups restored duplex stability to varying degrees with coumarin and psoralen significantly restoring stability.

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References

Glen Report 27.2: New Product - 1-Ethynyl-dSpacer-CE Phosphoramidite

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Notes

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: Compatible with all standard deprotection conditions. Best results are obtained with AMA, 65°C, 10 minutes.

Storage: Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 1-2 days

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