Transfection-EZ Biosystems: Primary Cells
Avalanche®- primary cell type-specific Transfection Reagent series?
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
If you work on cell lines rather than primary cells then click HERE
Human Preadipocyte (HPAd) Avalanche™ Transfection Reagent
Transfection of primary cells, such as HPAd, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
Human Preadipocyte (HPAd) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HPAd. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HPAd. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Preadipocyte (HPAd) Avalanche™ Transfection Reagent for transfection on HPAd
EZ Biosystems
Description
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Cell Information:
Human Preadipocytes (HPAd) are derived from human adipose tissue. These fibroblast-like precursor cells can be differentiated into Human Adipocytes (HAd). HPAd derived HAd were used to study insulin-stimulated glucose transport, growth hormone enhanced lypolysis and obese gene expression. Significant differences in lipolysis and leptin production are observed in HPAd and HAd from human omental and subcutaneous adipose tissue. HPAd/HAd are a useful in vitro cellular model for investigating the causes and treatment of obesity, type II diabetes and cardiovascular diseases. Recently it was discovered that human adipocytes might be a new target for the immunodeficiency virus.
The Transfection Reagent:
Transfection of primary cells, such as HPAd, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
Human Preadipocyte (HPAd) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HPAd. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HPAd. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Preadipocyte (HPAd) Avalanche™ Transfection Reagent for transfection on HPAd. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into HPAd
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Deliver single or multiple plasmids
- synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human preadipocytes after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human preadipocytes. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Preadipocyte Avalanche Transfection Reagent. |
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For Other Cells: |
Human Preadipocyte (HPAd) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.
3T3-L1
3T3-F442A
Ob17
AP-18
BJ Cell
Detroit 551 Cell
MG-63 Cell
Human Dermal Fibroblast (HDF)
Human Cardiac Fibroblasts (HCF, NHCF-A, NHCF-V, AoAF)
Human Lung Fibroblast (HLF, NHLF)
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200