Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Total DNA

Fast and easy methods for the micro scale purification of total (genomic and mitochondrial) DNA from various biological samples.

Allspin™

Allspin™ total DNA/RNA purification kit provides a convenient method for the isolation of total DNA and total RNA simultaneously from a single sample of tissue or cultured cells. DNA and RNA are purified separately from a same sample by individual but successive procedure using column B and column W respectively. more details of the procedure in the product desciption

GeneAll

Description

Description

The AllspinTM total DNA/RNA purification kit provides a convenient method for the isolation of total DNA and total RNA simultaneously from a single sample of tissue or cultured cells. DNA and RNA are purified separately from a same sample by individual but a successive procedure using column B and column W respectively. Whole procedure can be performed in just 30 minutes. The maximum length DNA obtainable is 50 kb (average 30 kb) and that of RNA is longer than 200 nucleotides.

 

Features and Benefits

Glass fibre membrane technology
Sample size : Up to 20 mg tissue or up to 1 x 107 cultured cells


Typical yield of RNA : Up to 20ug / 1 x 106 cultured cells Up to 60 ug / 10 mg liver tissue


Typical yield of DNA : Up to 10 ug / 1 x 106 cultured cells Up to 25 ug / 10 mg liver tissue

High purity
Preparation time : ~ 30 minutes
Stable and consistent yield
No phenol / chloroform extraction

No ethanol precipitation
Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular Cloning, real-time PCR, RNase protection assays and other analytical procedures

Click here for protocol

 

 

 

 

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Protocols

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References

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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200