qPCR and qRT-PCR: IG® PCR and qPCR Enzymes, Master Mix, and Kits

IG® PCR and qPCR Enzymes, Master Mix, and Kits

ntact Genomics (IG®, ig®) develops and manufactures high-quality enzymes, master mixes, and kits for conventional PCR, high-fidelity PCR, quantitative PCR (qPCR), genotyping, direct PCR, and nucleic acid sample preparation.

Our portfolio includes dye-based and probe-based qPCR master mixes, high-fidelity DNA polymerases, Taq and hot-start Taq formulations, ready-to-use PCR master mixes, Exonuclease I, and FastAmp® sample-preparation and direct-PCR solutions. These products are designed to deliver reliable amplification, high sensitivity and specificity, consistent performance, and convenient workflows across a broad range of research and molecular-testing applications.

Why Choose IG® PCR and qPCR Products?

Reliable Performance – Optimized for consistent amplification, sensitivity, specificity, and reproducibility.
Solutions for Diverse Applications – Products for routine PCR, high-fidelity amplification, qPCR, genotyping, direct PCR, and post-PCR primer cleanup.
Convenient Formats – Choose individual enzymes or ready-to-use master mixes to simplify setup and reduce pipetting errors.
Streamlined Sample Preparation – FastAmp® solutions enable rapid sample lysis and direct amplification without conventional DNA or RNA purification.
Consistent Quality – Manufactured under an ISO 13485:2016-certified quality management system.
Flexible Supply Options – Bulk quantities, custom formulations, private-label products, and OEM partnerships are available upon request.

Explore our PCR and qPCR product portfolio(opens in new tab) to select the right enzyme, master mix, or kit for your application.

Product Links	Size Available	Application & Benefits
IG® Green qPCR 2X Master Mix	200, 500 or 2,000 Reactions	Ready-to-use qPCR cocktail containing all components except primers and template.
igScript™ Probe-Based qPCR Master Mix	500, 1000, or 2,500 Reactions	Provides improved PCR efficiency, wider dynamic range, superior sensitivity, and specificity.
i7® High-Fidelity DNA Polymerase	200 or 500 Units	High fidelity, sensitivity, and processivity with an error rate approximately 280× lower than Taq DNA Polymerase.
i7® High-Fidelity DNA Polymerase 2X Master Mix	100 or 500 Reactions	Convenient master mix format of our high-fidelity polymerase.
Pfu 2X Master Mix	200 Reactions	Affordable and robust high-fidelity polymerase.
Taq DNA Polymerase	1,000 or 5,000 Units	For robust and affordable PCR.
Taq DNA Polymerase 2X Premix	500 or 1,000 Reactions	Convenient master mix format of our robust Taq DNA Polymerase.
Hot Start Taq 2X Master Mix	500 Reactions	Reduces non-specific amplification during setup and improves PCR specificity and sensitivity.
Exonuclease I (Exo I)(opens in new tab)	5,000 Units or 20,000 Units	Removes residual primers while preserving dsDNA.
FastAmp® Plant Direct PCR Kit	250 or 1,250 Reactions	Amplifies DNA directly from plant samples without DNA purification.
FastAmp® Plant Direct PCR & Genotyping Solution	20 mL	Rapid cell lysis and DNA separation without the need for DNA extraction.
FastAmp® Viral and Cell Lysis Solution	100 Reactions (50 mL) or 500 Reactions (250 mL)	Streamlines sample collection, storage, and amplification by isothermal RPA.

IG® Green qPCR 2X Master Mix

IG SYBR Green qPCR 2× Master Mix delivers consistent, reproducible results for all your gene expression and quantification assays. Main benefits include: Enhanced efficiency, specificity, and sensitivity Compatible with all major real-time PCR instruments Superior gene expression results under various cycling conditions Ready-to-use convenience More affordable price

Intact Genomics

Catalogue No.	Description	Pack Size	Price	Qty	Change to:
IG-3354	IG® Green qPCR 2X Master Mix	200 Reactions, 4x500 µl	£165.00	Quantity	Add
IG-3356	IG® Green qPCR 2X Master Mix	500 Reactions, 10x500 µl	£298.00	Quantity	Add
IG-3357	IG® Green qPCR 2X Master Mix	2000 Reactions, 40x500 µl	£849.00	Quantity	Add
IG-3360	IG® Green qPCR 2X Master Mix	2000 Reactions, 4x5ml	£760.00	Quantity	Add
IG-3362	IG® Green qPCR 2X Master Mix	5000 Reactions, 10x5ml	£1,470.00	Quantity	Add

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IG® Green qPCR 2X Master Mix

IG SYBR Green qPCR 2× Master Mix delivers consistent, reproducible results for all your gene expression and quantification assays. Main benefits include:

Enhanced efficiency, specificity, and sensitivity
Compatible with all major real-time PCR instruments
Superior gene expression results under various cycling conditions
Ready-to-use convenience
More affordable price

Product Description

Intact Genomics (IG®) Green qPCR 2x master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The IG® Green qPCR 2x master mix with integrated chemically modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye*, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high-quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

Applications

Gene expression data validation.
Absolute quantification
Mutation detection
Pathogen detection
viral detection
Genetically modified organisms (GMO) characterization
Genetic profiling

Product Includes

ig™ SYBR Green qPCR 2x Master Mix

Storage Temperature

–20 °C

Note

*The use of ROX dye is necessary for all Applied Biosystems instruments and is optional for the Stratagene Mx3000P™, Mx3005P™, and Mx4000™ cyclers. Bio-Rad, Qiagen, Eppendorf, Illumina and Roche instruments do not require ROX dye. The concentration of ROX Dye is (5 nM) for this product.

Technical Support & Customer Services
Intact Genomics (IG®) is dedicated to customer satisfaction regarding the use of our products for your research needs. Each new lot of our products is thoroughly tested to ensure it meets high quality standards and provides excellent results. We appreciate your business and your feedback regarding the performance of our products in your applications.

Please follow this protocol closely including the initial denaturation time and temperature.

Starting quantity of DNA can be 10pg-10ng. As little as 1pg may be acceptable with appropriate controls in place.

1. Place kit components, primers and RNA samples on ice.
2. Mix and then centrifuge briefly to collect contents at the bottom of the tube.
3. Prepare a master mix for each reaction and control plus 10% extra to allow for pipetting error according to the following table:

4. Mix the reaction mixture thoroughly.
5. Program the thermal cycler according to the manu-facturer’s instructions.
6. A typical PCR cycling program is outlined in the following table.

7. Place the PCR tubes in the thermal cycler and start the cycling program.
8. Analyze the data according to manufacturer protocol.
** For 3 step cycling protocols, anneal at optimal annealing temperature for 30 sec followed by the minimum time required for data acquisition at 72 ºC according to instrument guidelines.

Downloads

Manual – ig™ SYBR Green qPCR 2X Master Mix
MSDS – ig SYBR Green qPCR 2x Master Mix

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

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qPCR and qRT-PCR: IG® PCR and qPCR Enzymes, Master Mix, and Kits

IG® PCR and qPCR Enzymes, Master Mix, and Kits

Why Choose IG® PCR and qPCR Products?

IG® Green qPCR 2X Master Mix

Intact Genomics

IG® Green qPCR 2X Master Mix

Product Description

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