The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample. QuickExtract Solution has been used to extract DNA from samples such as hair follicles, quill-end cells of feathers, tissue-culture cells, buccal cells, zebrafish organs and scales, and mouse tail snips. The extracted DNA is suitable for PCR analysis, such as genomic, transgenic, or viral DNA screening in animals, or for genetic or environmental research and screening in humans and other organisms.

The QuickExtract method allows for the inexpensive processing of one to hundreds of samples simultaneously, without centrifugation, spin columns or the use of any toxic organic solvent. The method is also compatible with robotic automation.

IMPORTANT NOTE FOR QUICKEXTRACT USERS:

All end-users should bear in mind that QuickExtract Solutions generate crude extracts for PCR.  As such many types of PCR inhibitors contained in the original sample will also be present in the crude extract and it is essential to fully control for false negative results in any downstream assays.  Furthermore, it is debatable whether all possible types of PCR inhibition can be controlled for adequately using any previously described controls.

It is important to note that BioSearch Technologies/Lucigen/Epicentre QuickExtract Solutions (QuickExtract DNA and QuickExtract RNA) supplied by Cambio Ltd are not validated for use in diagnostic testing (including for SARS-CoV-2).  LGC Biosearch and Cambio Ltd take no responsibility for any results obtained. QuickExtract solutions are authorized for Research Purposes ONLY.

Resources

Rapid RNA extraction protocols for SARS-Cov-2 detection

Cui Lab - Washington University -  Rapid QuickExtract method

Feng Zhang Lab, Broad Institute – Rapid QuickExtract method

Latest Poster available

QuickExtract – Rapid and efficient extraction of PCR-ready genomic DNA from plant and seed samples

Figure 1. Procedure for obtaining PCR-ready DNA using QuickExtract™ DNA Extraction Solution.

Figure 2. FailSafe™ PCR amplifications of genomic DNA extracted from a variety of tissues or cells. Buccal cells were extracted using the BuccalAmp™ DNA Extraction Kit, and all other samples with QuickExtract™ DNA Extraction Solution. PCR was performed using primers to amplify the regions indicated: Lanes 1-3, human β-globin; lane 4, transgenic mouse GAPDH; lane 5, E. coli 16S ribosomal RNA gene; lane 6, transgenic SV40 T antigen.

Figure 3. Extracted DNA from multiple Zebrafish organs using QuickExtract™ DNA Extraction Solution 1.0. A 1-µl aliquot of a 100-µl extracted sample was used to amplify a single-copy crystallin-like gene. Lane 1, 100-bp ladder; lanes 2-3, fins; lanes 4-5, eyes; lanes 6-7, scales; lane 8, no-DNA control.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Epicentre’s product page, where the latest protocol is available.

Please note this will open a new page or window on your computer.

QuickExtract™ DNA Extraction Solution

(catalogue number QE0905T /QE09050)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Latest Poster available

QuickExtract – Rapid and efficient extraction of PCR-ready genomic DNA from plant and seed samples

References:

 Groschel, S.,Sanders, M., Hoogenboezem, R., de Wit, E., Bouwman, B., & Erpelinck, C. et al.(2014). A single Oncogenic Enhancer Rearrangement causes concomitant EVI1 and GATA2 Deregulation in Leukemia. cell, 157(2), 369-381.

 Ran, F., Hsu, P., Wright, J., Agarwala, V., Scott, D., & Zhang, F. (2013). Genome engineering using the CRISPR-Cas9 system. Nature protocols, 8(11), 2281-2308.

 Munoz-Cadavid, C., Rudd, S., Brandt, M., & Gomez B. (2010). Improving Molecular detection of fungal DNA in Formalin-Fixed paraffin-embedded tissues: Comparison of five tissue DNA Extraction methods using panfungal PCR. Journal of Clinical Microbiology, 48(6), 2147-2153.

 Carbery, I. D., et al. (2010) Targeted Genome Modification in Mice Using Zinc-Finger Nucleases. Genetics 186 , 451-459.

 Carilla-Latorre, S., et al. (2010) MidA is a putative methyltransferase that is required for mitochondrial complex I function. J Cell Sci 123 , 1674-1683.

 DeKelver, R. C., et al. (2010) Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome. Genome Res 20 , 1133-1142.

 Kotov, A. A. & Taylor, D. J. (2010) A new African lineage of the Daphnia obtusa group (Cladocera: Daphniidae) disrupts continental vicariance patterns. J Plankton Res , fbq018.

 Romeralo, M., et al. (2010) Two species of dictyostelid cellular slime molds from Alaska. Mycologia 102 , 588-595.

 Maloney B, Ray B, Hayden EP, Nurnberger JI Jr, Lahiri DK. Development and validation of the high-quality 'rapid method for swab' to genotype the HTTLPR serotonin transporter (SLC6A4) promoter polymorphism. psychiatr Genet. 2009; 19(2):72-82.

 Choi, J.-j., et al. (2009) Peptide Nucleic Acid-Based Array for Detecting and Genotyping Human Papillomaviruses. J Clin Microbiol 47 , 1785-1790.

 Macaluso, A. L., et al. (2009) Direct Effects of UV-B Radiation on the Freshwater Heterotrophic Nanoflagellate Paraphysomonas sp. Appl Envir Microbiol 75 , 4525-4530.

 Van Driel, L. M. J. W., et al. (2009) Body Mass Index Is an Important Determinant of Methylation Biomarkers in Women of Reproductive Ages. J Nutr 139 , 2315-2321.

 Van Driel, L. M. J. W., et al. (2008) Eight-fold increased risk for congenital heart defects in children carrying the nicotinamide N-methyltransferase polymorphism and exposed to medicines and low nicotinamide. Eur Heart J 29 , 1424-1431.

 Viola, A. U., et al. (2008) PER3 polymorphism and cardiac autonomic control: effects of sleep debt and circadian phase. Am J Physiol Heart Circ Physiol 295 , H2156-2163.

 Dos Santos, P. C., et al. (2007) Controlled Expression of nif and isc Iron-Sulfur Protein Maturation Components Reveals Target Specificity and Limited Functional Replacement between the Two Systems. J Bacteriol 189 , 2854-2862.

 Hitchins, M. P., et al. (2007) Inheritance of a Cancer-Associated MLH1 Germ-Line Epimutation. N Engl J Med 356 , 697-705.

 Kotov, A. A., et al. (2006) A new species in the Daphnia curvirostris (Crustacea: Cladocera) complex from the eastern Palearctic with molecular phylogenetic evidence for the independent origin of neckteeth. J Plankton Res 28 , 1067-1079.

 Ward, K. N., et al. (2006) Human Herpesvirus 6 Chromosomal Integration in Immunocompetent Patients Results in High Levels of Viral DNA in Blood, Sera, and Hair Follicles. J Clin Microbiol 44 , 1571-1574.

 Hsu, C., et al. (2005) Primary Human T Lymphocytes Engineered with a Codon-Optimized IL-15 Gene Resist Cytokine Withdrawal-Induced Apoptosis and Persist Long-Term in the Absence of Exogenous Cytokine. J Immunol 175 , 7226-7234.

 Bough, K. J., et al. (2004) Medial Perforant Path Inhibition Mediated by mGluR7 Is Reduced After Status Epilepticus. J Neurophysiol 92 , 1549-1557.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

IMPORTANT NOTE FOR QUICKEXTRACT USERS:

All end-users should bear in mind that QuickExtract Solutions generate crude extracts for PCR.  As such many types of PCR inhibitors contained in the original sample will also be present in the crude extract and it is essential to fully control for false negative results in any downstream assays.  Furthermore, it is debatable whether all possible types of PCR inhibition can be controlled for adequately using any previously described controls.

It is important to note that BioSearch Technologies/Lucigen/Epicentre QuickExtract Solutions (QuickExtract DNA and QuickExtract RNA) supplied by Cambio Ltd are not validated for use in diagnostic testing (including for SARS-CoV-2).  LGC Biosearch and Cambio Ltd take no responsibility for any results obtained. QuickExtract solutions are authorized for Research Purposes ONLY.

Resources

Rapid RNA extraction protocols for SARS-Cov-2 detection

Cui Lab - Washington University -  Rapid QuickExtract method

Feng Zhang Lab, Broad Institute – Rapid QuickExtract method

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications

• Simple, rapid extraction of PCR-ready DNA for transgenic mouse genotyping, genetic studies, human identity testing, or viral/microbial screening.

Benefits

• Nontoxic reagents, inexpensive processing.
• Short procedure (8 minutes for the average sample).
• No centrifugation or spin columns to reduce yields.
• Compatible with high-throughput and robotic workflows

Latest Poster available

QuickExtract – Rapid and efficient extraction of PCR-ready genomic DNA from plant and seed samples

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200