QUESTION: Please send me more information regarding the 3' spacer columns that will protect against exonuclease and polymerase activity?
RESPONSE:We have no definitive data on the propyl CPG to support the assertion that it protects oligos from exonuclease digestion and does not permit polymerase extension. Our conclusion is based by analogy to the propylamino-modifier CPG(1) (Cat. No. 20-2950-41). This modification protects oligos from exonuclease digestion but permits polymerase extension to a small extent since the modifier is eliminated to a level of about 10% from the 3' terminus, leaving the 3'-hydroxyl group available. HPLC experiments have shown that there is no detectable elimination of the propyl group from oligos made from the spacer C3-CPG.
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