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PCR

PCR : PCR Products

The FailSafe™ PCR system for templates up to 20kb long, high GC content (up to 85% G+C), & Multiplex PCR


Aline PCRClean DX

Are you looking for a direct replacement for Beckman Ampure XP? PCRClean DX is a high quality reagent with a fraction of cost.

Aline Biosciences

Catalogue No.DescriptionPack SizePriceQty
AL-AC1003-250Aline PCRCLEAN DX250ml POA Quantity Add to Order
AL-AC1003-450Aline PCRCLEAN DX450ml POA Quantity Add to Order
AL-AC1003-5Aline PCRCLEAN DX5ml £104.00 Quantity Add to Order
AL-AC1003-50Aline PCRCLEAN DX50ml £406.00 Quantity Add to Order

Description

PCRClean DX

 

Product Overview:

The PCRClean DX purification system utilizes Aline’s proprietary paramagnetic bead technology for rapid high-throughput purification of DNA fragments.

The consistent recovery efficiency of the PCRClean DX for small and large amplicon sizes is superior to traditional filtration PCR cleanup methods. As such, the PCRClean DX is designed to directly replace Beckman Coulter and Agencourt’s Ampure XP without any protocol changes while providing end-users with superior, more efficient adaptor removal in NGS Library Prep and primer-dimer removal.

 

Product Highlights

    • Flexible clean-up: high recovery of amplicons with option to choose recovery of >100 bp or >60 bp with modified protocol
    • Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
    • Processing time: 15 minutes/96 samples
    • Scalable: suitable for tube, 96 and 384-well plate formats
    • Compatible with all next generation sequencing platforms (e.g., Illumina, Ion Torrent, Roche’s 454)

Applications:

  • Sanger sequencing post-PCR cleanup
  • Genotyping and mutation detection
  • DNA Enriching/Concentrating
  • Primer Walking
  • All other downstream molecular engineering

Datasheet

Click here

PCRClean DX pics on web_1.comparison

PCRClean DX pics on web_2.comparison

** For product references in research and publications, see the Product Resources tab.

 

izes:

  • 5 mL, 500 – 1,000 preps
  • 50 mL, 5,000 – 10,000 preps
  • 250mL, 25,000 – 50,000 preps
  • 450 mL, 45,000 – 90,000 preps

Storage:

  • Store beads at 2-8°C, DO NOT FREEZE
  • Store all other components at 15-25°C

Stability:

  • 12 months if stored as specified

 

Disclaimer: For research use only. Not for use in diagnostic procedures.

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Protocols

 

Datasheet -  click here

 

MSDS - click here

 

Protocol click here

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

References

Reference Journal Publication(s)

Sun et al. Proceedings of the National Academy of Sciences of the United States of America, 2012

http://www.pnas.org/content/110/9/E808.full.pdf html

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

  • Next Generation Sequencing platforms, Illumina, Ion Torrent, MinION, Roche/454
  • Sanger sequencing post-PCR cleanup
  • Genotyping and mutation detection
  • DNA Enriching/Concentrating
  • Primer Walking
  • All other downstream molecular engineering

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200