Cambio - Excellence in Molecular Biology

Molecular Cloning Kits

Molecular Cloning Kits

The products in this section are designed for molecular cloning of cDNA, gene, PCR fragments (up to 15kb) and also the construction of libraries for much larger fragments (up to 40kb) such as fosmids. Kits are available with both electrocompetent and chemically competent cells. In regards to vector and insert preparation, products in this section enable recovery of nucleic acids from agarose gels. Sheared DNA can be repaired and then ligated for blunt-end cloning, genomic DNA cloning and subcloning.

Molecular Cloning Kits and associated competent cells

Our molecular cloning reagents enable rapid, efficient and accurate, cloning and library construction through the use of well-established and characterised electrocompetent and chemically competent cells together with excellent tried and tested kits for ligation as well as fosmid construction.


CopyControl™ Fosmid Library Production Kits

With this kit end users can not only prepare complete and unbiased fosmid libraries, from any biological sample but they can also maintain clones at a single-copy number and induce them to high-copy number as needed.

FosmidMAX™ DNA Purification Kit

Easy, reliable isolation of high-quality fosmid DNA.  Isolate up to 25 µg 100ml cultures of a single-copy fosmid, with an easy to use protocol. There is no use of columns or binding resins (that reduce yield) in this kit which makes the procedure fully scalable.


TransforMax™ EPI300™ Electrocompetent and Chemically Competent E. coli

An established cell line that can be used for standard cloning of large inserts and Gibson assembly electroporation. Cloning of large inserts and transformation of large plasmids up to 145 kb are within easy reach.  


End-It™ DNA End-Repair Kit

Easily Convert DNA with damaged or incompatible protruding ends into ready-to-ligate, 5´-phosphorylated, blunt-ended DNA. Ideal for repairing sheared, nebulized or restriction digested enzymes


Fast-Link™ DNA Ligation Kit

Using a high-quality ligase formulated to provide extremely rapid, high-efficiency DNA ligation, this kit can perform cohesive-end ligations in an impressive 5 minutes. A whole range of applications from blunt end repair to library construction are possible.


Ampligase® Thermostable DNA Ligase

With its highly thermostable DNA ligase, this kit is suitable for applications where ligation at high temperature or high hybridisation stringency washes pose a benefit. It has been shown to be active for at least 500 thermal cycles (94°C/80°C) or 16 hours of cycling.


T4 Polynucleotide Kinase, Cloned

Highly active enzyme that readily phosphorylate 5'-hydroxy ends of ssDNA and dsDNA, RNA, and nucleoside 3´ monophosphate. The enzyme also removes the 3´-phosphates from various species of nucleosides click here for details.