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PCR

PCR

The PCR products in this section cover a wide range of PCR technologies. Here you will find products suitable for both TA and blunt-end cloning, for high fidelity PCR, PCR on GC-rich regions and long range PCR. You can find here: PCR mastermixes to optimise and streamline your current PCR methods. The FailSafe PCR system allows for rapid optimisation of any PCR in one single run, thanks to the buffer sets which have already been optimised with the correct pH, amounts of magnesium and PCR enhancers.

PCR Master Mixes & Amplification Systems

 

Cambio supply ready-to-use PCR formulations from many high-end molecular biology suppliers.  Our kits and master mixes are designed to improve reproducibility, reduce pipetting errors, and streamline workflow.  Whether you are looking to do standard, high performance or specialist PCR we have the right kit for you.  The range includes

  • Standard PCR master mixes
  • High-fidelity PCR master mixes
  • Long-range PCR mixes
  • GC-rich template PCR mixes
  • RT-PCR and cDNA synthesis systems
  • TA cloning PCR systems
  • Blunt-end cloning PCR systems

Notable Products

FailSafe™ PCR System

  • Rapid PCR optimisation platform
  • Multiple pre-optimised buffer systems
  • Optimised pH, magnesium concentrations and enhancers
  • Enables challenging PCR targets to be amplified with minimal optimisation effort.

High-Fidelity & Specialist PCR Enzymes

PCR is fundamental technique in molecular biology so why compromise on the key enzymes for involved in this process? Cambio provides specialist PCR enzymes designed for applications demanding accuracy and robustness.

Applications

  • Cloning
  • Sequencing
  • Mutation analysis
  • Amplification of difficult templates
  • GC-rich target amplification

Includes

  • High-fidelity DNA polymerases
  • Enhanced PCR enzyme systems
  • Speciality amplification reagents

PCR Reagents & Optimisation Components

If you require supporting reagents for assay development and troubleshooting then Cambio can accommodate your needs.  We supply :-

  • PCR buffers
  • Reaction enhancers
  • dNTPs
  • Additives for difficult templates
  • General amplification reagents

These can be used to  increase specificity, yield, sensitivity and reproducibility.


DNA Extraction for PCR

QuickExtract™ DNA Solution

Our best-selling product for the last 20+ years !! and there is a very good reason for this…

The  QuickExtract™ DNA Solution is a simple but fully scalable and highly effective DNA extraction reagent that generates PCR-ready DNA directly from any biological samples in as little as 8 minutes. No complicated protocols and no specialist equipment needed.  Sample types include but not limited to buccal swabs, hair follicles, mouse tissue samples, Zebrafish embryos, cultured cells


PCR Clean-Up & Post-PCR Processing

PCRClean DX™

A very effective magnetic bead purification system based on paramagnetic bead technology.  The PCRClean DX™ is a direct cost-effective replacement for Beckman’s AMPure XPTM with no protocol changes required. It removes Unincorporated primers, primer dimers, dNTPs, Salts and other PCR contaminants. Use this kit for:-

  • NGS library preparation
  • Sanger sequencing
  • Genotyping
  • Mutation detection
  • Post-PCR purification

Residual DNA Testing

PCR-based solutions for quality control and regulated testing environments.

Applications

  • Biopharmaceutical manufacturing
  • Host-cell DNA quantification
  • Regulatory compliance testing
  • Process validation

Supports DNA contamination monitoring and quality assurance workflow