Cambio - Excellence in Molecular Biology

Gene Expression Analysis

Gene Expression Analysis

The products in this section are used in gene expression analysis. Specific applications include generation of ds DNA from small RNAs for high through-put (next-gen) sequencing templates, labelled small RNA for microarray analyses, identification of 5'' and 3'' eukaryotic mRNA, identification of exact transcription start sites and creation of cDNA libaries that represent full-length selected transcripts from total RNA or enriched mRNA. The TargetAmp Kits produce microgram amounts of aRNA form mRNA in total RNA and can incorporate both aminoallyl and biotin labels if desired.

Product Highlights 

TargetAmp aRNA Amplification


DNA microarrays provide a powerful method to measure the abundance of thousands of different RNA molecules in a single experiment. The amount of RNA required for gene expression analysis, however, can be a limiting factor when the available biological sample is small, as in the case of needle biopsies or laser capture micro-dissected cells. RNA amplification technology has made transcription profiling of such small samples feasible. 

TargetAmp aRNA kits utilises a significantly improved 'Eberwine-type' aRNA amplification protocol, that generates microgram quantities of amplified antisense RNA from picogram amounts of total RNA. This represents at least 5,000,000-fold amplification of the polyadenylated component of the original total RNA, making microarray analysis of single cells possible.  The aRNA produced can be labelled with biotin or fluorescent dyes, and performs well on multiple microarrays.


The MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit 


The MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit, is optimized for generating full-length first-strand cDNA from total cellular RNA preparations or purified polyadenylated RNA. 

MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit includes Epicentre's high activity native MMLV reverse transcriptase and an optimized, proprietary Reaction Buffer. A kit reaction is capable of synthesizing 1st-strand cDNA from RNA templates longer than 15 kb.


The MessageBOOSTER cDNA Synthesis Kit for qPCR


The MessageBOOSTER cDNA Synthesis Kit for qPCR enables the user to perform hundreds of sensitive qRT-PCR amplifications from very small amounts of total RNA… even total RNA from as little as one eukaryotic cell. The kit amplifies the poly(A) RNA in a total RNA sample and then converts the amplified RNA to cDNA that is ready for PCR or qPCR. The MessageBOOSTER cDNA Synthesis Kit for qPCR uses oligo(dT) to prime cDNA synthesis. Therefore, this kit is best used with intact total RNA preparations.

MessageBOOSTER is not suited for bacterial/prokaryotic total RNA samples