These molecular cloning products have high efficiency for DNA ligations and high throughput DNA cloning. Readily clone PCR or RT-PCR products and accomplish a wide range of DNA engineering tasks.
Product Highlights
CopyControl™ Fosmid Library Production Kits
BioSearch Technologies (Lucigen/Epicentre)
CopyControl™ Fosmid Library Production Kits provide an efficient and improved method for constructing a library of cosmid-sized (approximately 40kb) clones. The CopyControl™ Fosmid Library Production Kit contains the original pCC1FOS™ Vector, while the recently introduced CopyControl™ HTP Fosmid Library Production Kit includes a modified pCC1FOS Vector, called pCC2FOS. Both of these CopyControl™ Vectors contain both the E. coli F-factor single-copy origin of replication and the inducible high-copy oriV. CopyControl™ Fosmid clones are grown at single copy to ensure insert stability and successful cloning of encoded and expressed toxic protein. The CopyControl™ Fosmid clones can then be induced up to 50 copies per cell.
CopyCutter™ EPI400™ E.coli* cells
BioSearch Technologies (Lucigen/Epicentre)
CopyCutter™ EPI400™ E.coli* cells were developed to significantly lower the copy number of a wide variety of common vectors so that you can more readily clone unstable DNA sequences. DNA that is unstable at high-copy number often codes for a protein that inhibits cell growth or contains AT- and GC-rich sequences or sequences with strong secondary structure. The CopyCutter™ EPI400™ cell line was derived from our high-transformation efficiency phage T1-resistant TransforMax™ EC100™ E. coli strain by manipulating a gene that controls the copy number of vectors containing ColE1 or pMB1 origins of replication (e.g., pUC- and pET-type vectors).
MaxPlax™ Lambda Packaging Extract
BioSearch Technologies (Lucigen/Epicentre)
MaxPlax™ Lambda Packaging Extract offers maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E. coli strains, BHB2690 and BHB2688, as described by Hohn. MaxPlax™ Extract utilises a restriction-free E. coli K-12 strain, NM759, in place of strain BHB2690. The sonicated extract of NM759 is combined with an extract of BHB2688 to produce MaxPlax™ Extract.