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Oligo Synthesis

Oligo Synthesis : CEPs

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5'-Carboxy-Modifier C5

2-chlorotrityl 4-[(2-cyanoethyl)-(N,N-diisopropyl)phosphoramidityl]pentanoate

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1945-025'-Carboxy-Modifier C50.25g £350.00 Quantity Add to Order
10-1945-905'-Carboxy-Modifier C5100umole £100.00 Quantity Add to Order

Description

5'-Carboxy-Modifier C5

 

 

Product Specifications

 

Formula:
C33H40ClN2O4P
M.W.:
595.11
F.W.:
180.1
CAS Number:
486449-23-4

 

Description
The disulfide thiol modifier may be used for introducing 3’- or 5’-thiol linkages. Dithiol Serinol, produced from lipoic acid and our patented serinol backbone, allows easy connection of multiple dithiol-labelled oligos to gold surfaces. 5’-Carboxy-Modifier C10 is a unique linker designed to be added at the terminus of an oligonucleotide synthesis. It generates an activated carboxylic acid N-hydroxysuccinimide (NHS) ester suitable for immediate conjugation on the synthesis column with molecules containing a primary amine, resulting in a stable amide linkage. An alternative carboxylate protecting group is the 2-chlorotrityl group, which is simply removed using the standard deblock cycle to generate a free carboxyl group on an otherwise fully protected oligonucleotide. The 2-chlorotrityl group is also removed during oligo deprotection with ammonium hydroxide or AMA and is incompatible with RP purification techniques. PC Amino-Modifier is a photocleavable C6 amino-modifier, part of our line of photocleavable (PC) modifiers. 5’-AminoOxy-Modifier 11 is based on a tetraethylene glycol linkage for improved solubility and for reducing the potential negative impact on hybridization of the oligo. The oxime formed from the reaction of alkyloxyamines with aldehydes creates a stable covalent bond. In comparison, the imine formed by the conjugation of primary amines with aldehydes is not stable to acidic or basic conditions and requires subsequent reduction with borohydride to form stable amine conjugates. 5’-Maleimide Modifier Phosphoramidite, developed at the University of Barcelona, incorporates a maleimide cycloadduct that is stable to ammonium hydroxide at room temperature. This phosphoramidite can be incorporated into DNA and RNA with both phosphate and phosphorothioate linkages. A retro–Diels-Alder reaction deprotects the maleimide immediately prior to conjugation.

 

Usage

  • Coupling: 3 minute coupling time recommended.
  • Deprotection: Deprotect as required by nucleobases. NOTE: the 2-chlorotrityl cannot be retained for reverse phase purification. 
Specifications
Diluent Anhydrous Acetonitrile
Recommended Storage Freezer storage, -10 to -30°C, dry
Stability In Solution 1-2 days

 

Dilution/Coupling Data
 

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of Additions

LV40LV20040nm0.2μm1μm10μm
10-1945-02 0.25 g .25grams 4.2 126.67 76 47.5 34.55 25.33 6.33
10-1945-90 100 µmol .06grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog #Pack SizeGrams/PackDilution (mL)Approximate Number of Additions

Molarity50nm0.2μm1μm15μm
10-1945-02 0.25 g .25grams 6.27 0.07 119 74.38 54.09 7.44
10-1945-90 100 µmol .06grams 1.5 0.07 23.6 14.75 10.73 1.48
Technical documents
Safety Data Sheet (North America)
View Safety Data Sheet
Safety Data Sheet (EU)
View Safety Data Sheet
Safety Data Sheet (Other)
View Safety Data Sheet

 

 

 

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Protocols

MSDS

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References

Glen Report 26.2

A.V. Kachalova, et al., Helv Chim Acta, 2002, 85, 2409-2416.

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Notes

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack Size Grams/ Pack 0.1M Dil. (mL) LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1945-02 0.25grams .25grams 4.2 126.67 76 47.5 34.55 25.33 6.33
10-1945-90 100µmoles .06grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack Size Grams/ Pack Dilution (mL) Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1945-02 0.25grams .25grams 6.27 .07 119 74.38 54.09 7.44  
10-1945-90 100µmoles .06grams 1.5 .07 23.6 14.75 10.73 1.48  
Mermade
Cat.No. Pack Size Grams/ Pack Dilution (mL) Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1945-02 0.25grams .25grams 6.27 .07 120.6 75.38 54.82    
10-1945-90 100µmoles .06grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200