ur PCR Quantification Standards contain genomic DNA extracted from defined microorganisms, at low culture passage number. The DNA starting material is analyzed with standardized methods to determine the DNA’s integrity and quantity. Only pure and intact DNA showing a > 10 kb band in an agarose gel and an OD260/280 > 1.8 is used for the manufacture of these products. The DNA is partially sequenced to confirm the species’ identity.

Recommended Use

PCR Quantification Standards contain a defined number of genome copies of a particular microorganism. Therefore, our standards are perfectly suitable to generate DNA standard curves in qPCR. In addition, standards are required for validating the specificity and testing the sensitivity of PCR assays in the context of quality control and EP 2.6.7 compliant-testing.

Content

1 vial with 1x10⁸ genomes (lyophilized)
3 vials with rehydration buffer (10 mM, pH 8.5)

Available Species and Ordering Information

• Cat. No. 52-0116 Acholeplasma laidlawii
• Cat. No. 52-1988 Aspergillus brasiliensis
• Cat. No. 52-0819 Aspergillus fumigatus
• Cat. No. 52-0347 Bacillus subtilis
• Cat. No. 52-1447 Bacteroides vulgatus
• Cat. No. 52-5571 Bordetella pertussis
• Cat. No. 52-1386 Candida albicans
• Cat. No. 52-1226 Candida glabrata
• Cat. No. 52-6128 Candida krusei
• Cat. No. 52-1953 Candida tropicalis
• Cat. No. 52-1664 Clostridium sporogenes
• Cat. No. 52-0083 Escherichia coli
• Cat. No. 52-0348 Kocuria rhizophila
• Cat. No. 52-0079 Lactobacillus acidophilus
• Cat. No. 52-0101 Legionella pneumophila
• Cat. No. 52-0129 Mycoplasma arginini
• Cat. No. 52-0117 Mycoplasma fermentans
• Cat. No. 52-0115 Mycoplasma gallisepticum
• Cat. No. 52-0130 Mycoplasma hyorhinis
• Cat. No. 52-0112 Mycoplasma orale
• Cat. No. 52-0119 Mycoplasma pneumoniae
• Cat. No. 52-0124 Mycoplasma synoviae
• Cat. No. 52-0103 Mycoplasma salivarium
• Cat. No. 52-0164 Spiroplasma citri
• Cat. No. 52-0848 Penicillium chrysogenum
• Cat. No. 52-0071 Pseudomonas aeruginosa
• Cat. No. 52-0799 Staphylococcus aureus

Shelf Life and Storage

The components must be stored between 2 and 8 °C (Shipping at ambient temperature).

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Protocol

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Dumke R. et al., (2017). Multi-center evaluation of one commercial and twelve in-house real-time PCR assays for detection of Mycoplasma pneumoniae. Diagnostic Microbiology & Infectious Disease, doi: 10.1016/j.diagmicrobio.2017.03.004.

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Applications

These DNA-Standards allow for the performance of a control reaction for PCR using qPCR instrumentation and to create a standard curve for quantification experiments, i.e. with Venor^®Mp Mycoplasma pneumoniae Diagnostic Kit for qPCR. The DNA standards can be used as a general control to confirm PCR system performance using a qPCR instrument or for the validation of detection limits in respect to conventional PCR tests.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200