Cambio's Star*FISH© system provides an excellent method for the identification of single human chromosomes, and allows the detection of translocations and insertions on metaphase chromosomes. Marker chromosomes can also be identified and complex karyotypes analysed.
Probes are available for all chromosomes. To allow users to undertake more ambitious protocols, paints are now supplied in concentrated form with separate hybridisation buffer. This allows up to three paint colours to be used per sample with no loss of signal or specificity. The individual paints come in packs of 10 and 20 tests. Stability is guaranteed for 12 months, and many paints have shown no loss of potency after 5 years if stored at -20°C.
All Cambio paint systems for whole human chromosomes are now Cot-1 free. Ground breaking work by our collaborators in the University of Cambridge has resulted in competitor DNA-free painting. Our paints have good, if not better, specificities and signal intensities than Cot-1 containing paints, provided they are used with the optimised Star*FISH© protocols and detection reagents.
ALTechnologies whole chromosome probes labelled with digoxigenin (DIG) are now available from Cambio. Please note that for chromosomes 13, 14, 15, 21 and 22 only the 'q' arm is labelled. The probe DNA is constructed from microdissected normal human metaphase chromosome DNA, then directly amplified by PCR using a degenerate primer.
Paraffin Sections
Recently, a paraffin protocol has been devised for use of chromosome paints with paraffin sections.
Protocol Listings
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200