Bz-5-Me-C-LA-CPG
5'-Dimethoxytrityl-N-benzoyl-5-methyl-(2'-O, 4'-C methylene)-Cytidine,3'-succinoyl-long chain alkylamino-CPG
Safety Data Sheet
Glen Report 34-23: New Products — LA CPGs
Locked Nucleic Acid (LNA) is a
modified ribonucleic acid containing a methylene bridge connecting the
2’-oxygen and 4’-carbon atoms. Oligonucleotides modified with (LNA), are
prominent for hybridization assays and probes due to enhanced thermal
stability towards complementary oligos, without compromising base
pairing specificity. Each LNA modification can increase the melting
temperature of a duplex by 2-8 °C. LNAs are commonly employed in
antisense oligonucleotides ASOs as gapmers or mixers.
Our locked analog or LA CPGs are designed to produce 3’-LNA modified
oligonucleotides. The pyrimidine bases are thymidine and
5-methylcytosine, rather than uracil and cytosine, respectively.
Details
Usage
- Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
- Deprotection:
No changes needed from standard method recommended by synthesizer
manufacturer. Note: Deprotection in AMA (50:50 Ammonium
hydroxide/MethylAmine) may result in a 4-5% 5-methyl-N4-methyl-C side
product at the modification site.
| Specifications |
| Storage |
Freezer storage, -10 to -30°C, dry |
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