INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix

• Reprogram fibroblasts with an optimized, proprietary mix of five pseudouridine (Ψ)-containing, dsRNA-depleted mRNAs.
• Ultra-low immunogenicity Ψ-mRNA mix effectively avoids recognition by cellular innate immune response systems.
• Reprogram fibroblasts with as few as five transfections and get colonies within two weeks.
• Have peace of mind and save on downstream QC knowing no residual vector remains after reprogramming.

Product is for research use only (RUO)

 

Use and Label Licenses for Specific Products: Purchaser receives a limited non-exclusive, non-transferable right to use the Products purchased from CELLSCRIPT™ solely for its own internal laboratory research use. See the Label License tab for more information. Click here for more information

 

Product Description:

The INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix contains an optimized, proprietary mix of five pseudouridine (Ψ)-containing, double-stranded RNA (dsRNA)-depleted mRNAs encoding proteins with the ability to reprogram somatic cells, specifically fibroblasts, into induced pluripotent stem cells (iPSCs) with high efficiency. INCOGNITO™ mRNAs contain the uridine analog pseudouridine which is substituted for all uridines in the mRNA sequences. This substitution combined with dsRNA removal using the Min-Immune™ Gold dsRNA Removal system produces a mRNA mix with ultra-low immunogenicity that effectively avoids recognition by the receptors of the cellular innate immune response system.

The INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix is highly efficient, requiring as few as five transfections and producing colonies within two weeks. Due to the transient nature of mRNA in cells, INCOGNITO™ mRNAs leave no residual vector after reprogramming and pose no risk of genomic integration of the exogeneous factors, reducing downstream quality testing.

The Reprogramming Mix is available to reprogram either 6 wells (Cat. No. IPY250906) or 12 wells (Cat. No. 2xIPY250906) of a 6-well plate.

Cells reprogrammed into iPSCs can be differentiated into multiple cell types including neurons, cardiomyocytes and pancreatic islet cells and can be a source of cells for use in basic science studies, drug safety screening, development of immunotherapies and regenerative medicine therapies.

Product Performance:

 

Figure 1. INCOGNITO™ iPSCMax™ Reprogramming Timeline. Cells are reprogrammed into iPSCs with as few as five daily transfections, generating colonies within two weeks.

 

Figure 2. Alkaline Phosphatase staining of iPSC colonies in a well of fibroblasts reprogrammed using the INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix. The Reprogramming Mix results in high efficiency reprogramming producing colonies within two weeks.

Figure 3. Cells reprogrammed using the INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix stained positive for several pluripotency markers.

Figure 4. Cells reprogrammed using the INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix differentiated into the three germ layers and stained positive for markers of each respective germ layer.

Product Resources

→ Manual

→ SDS

→ Product Flyer

 

Kit Contents

Materials Supplied:

Important Store at -70°C in a freezer without a defrost cycle. Do not store at -20°C.

 

 

 

Materials Required, but not Supplied

 

 

 

* Fibroblasts may be grown and expanded in any appropriate fibroblast growth/maintenance medium. The fibroblast medium available through ATCC is one suggestion.

** IMPORTANT: This protocol has been optimized using the Lipofectamine MessengerMAX transfection reagent. It is strongly recommended that this reagent be used for transfection without substitution.

*** Other substrates may also be used to coat plates for reprogramming including Matrigel^®, Vitronectin XF™, and CELLstart™ substrates.

 

LIMITED LABEL LICENSE

CELLSCRIPT™’s INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix (“Product”) is for rapid and efficient induction of induced pluripotent stem cells (iPSCs) from ex vivo human or mammalian fibroblasts.  This Product and the compositions, kits, systems, methods and/or uses thereof are covered by WIPO PCT Patent Application Numbers WO2007024708A2 and WO2011071931A2 and U.S. Patent Numbers US8278036B2 and US9750824B2, and U.S. and international divisional, continuation, or other patents and patent applications derived therefrom that are owned by the University of Pennsylvania and licensed to CELLSCRIPT™, and by U.S Patent Number US 9,580,689 B2, which is exclusively licensed to CELLSCRIPT™ by the University of Minnesota.

By purchasing Product from CELLSCRIPT™ or an authorized distributor of CELLSCRIPT™ (herein, “Purchaser”), Purchaser receives a limited non-exclusive, non-transferable, non-sublicensable right (“Limited License”) to use the purchased Product solely to generate iPSCs from ex vivo human fibroblast cells for its own internal laboratory research use (the “Licensed Use”) and Purchaser agrees to cite the name of the Product and CELLSCRIPT™ as its source in any verbal or written public disclosure about said Licensed Use.  The Licensed Use expressly excludes using Product for any Commercial Use or for making any product for Commercial Use, including: [A] for any diagnostic, prophylactic, clinical, therapeutic or other use in humans, [B] for any veterinary, livestock, or agricultural use in animals, [C] for generating human iPSCs, hematopoietic stem or progenitor cells, neurons, immune cells or any other cells, and/or [D] for the manufacture, distribution, importation, exportation, or sale of products and/or services made using Product for which Purchaser receives compensation of any kind, it being understood that [A]–[D] are separately and/or collectively defined as a “Commercial Use” herein.  No license rights are granted, expressly or implied, to make, have made, import, export, use, reverse engineer, transfer, offer for sale, or sell Product, derivatives of Product, or services using Product for a Commercial Use.  By purchasing Product, Purchaser agrees not to make, have made, import, export, use, reverse engineer, transfer, offer for sale, or sell Product, components of Product, or derivatives of Product, or to provide services, or to generate information, data, compositions, methods, applications, or uses thereof in exchange for money or any other consideration, and Purchaser agrees not to use Product, components of Product, or derivatives of Product to support filing of a patent application in any country or other patent office in the world unless otherwise agreed by CELLSCRIPT™ in writing prior to such use. CELLSCRIPT™ provides no warranties (statutory or implied) concerning merchantability or fitness of Product for a particular use and does not represent or warrant that Purchaser’s use of Product will not infringe patent or other intellectual property rights of any other party, and all such warranties of non-infringement are expressly disclaimed.  This Limited License terminates automatically if Purchaser breaches any of the terms herein.

This Limited License remains in effect if Product is being used in accordance with the license terms.  The license is terminated immediately upon user’s breach of any of the license terms, such as using the product for commercial purposes without proper authorization.

If your organization is interested in using INCOGNITO™ iPSCMax™ Ψ-mRNA Reprogramming Mix or any other CELLSCRIPT™ products for a Commercial Use, whether in catalog or custom sizes, please contact us with a description of your interest and needs.

 

 

 

 

 

 

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