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Oligo Synthesis

Oligo Synthesis : CEPs

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N4-Methyl-C-CE Phosphoramidite N4-Methyl-C-CE Phosphoramidite

5'-Dimethoxytrityl-N4-acetyl-N4-methyl-Cytidine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3016-02N4-Methyl-C-CE Phosphoramidite0.25grams £586.00 Quantity Add to Order
10-3016-90N4-Methyl-C-CE Phosphoramidite100µmoles £218.00 Quantity Add to Order
10-3016-95N4-Methyl-C-CE Phosphoramidite50µmoles £118.00 Quantity Add to Order

Description

N4-Methyl-C (m4C) modification has been identified in the bacterial cytoplasmic rRNA as well as in eukaryotic mitochondrial rRNA. In this modification, a methyl group is attached to the exocyclic amine of cytosine. Compared to unmodified C, m4C destabilizes base pairing.

 

Product Specifications

Formula: C48H66N5O9PSi
M.W.: 916.14
F.W.: 319.21
CAS Number: 1253796-11-0

 

Dilution/Coupling Data

 

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of Additions

LV40LV20040nm0.2μm1μm10μm
10-3016-95 50 µmol 0.05 0.5 3.3 2 1.2 0.9 0.6 0.1
10-3016-90 100 µmol 0.09 1 20 12 7.5 5.4 4 1
10-3016-02 0.25 g 0.25 2.73 77.6 46.6 29.1 21.1 15.5 3.8

Expedite

Catalog #Pack SizeGrams/PackDilution (mL)Approximate Number of Additions

Molarity50nm0.2μm1μm15μm
10-3016-02 50 µmol 0.05 1 0.7 8.6 5.3 1.7 0.5
10-3016-90 100 µmol 0.09 1.5 0.7 23.6 14.7 4.9 1.4
10-3016-95 0.25 g 2.5 4.07 0.7 75 46.8 15.6 4.6

 

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References

Glen Report 37.2

TBDMS-protected RNA guide

SDS

Note that links go to manufacturer's website

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Applications & Benefits

Usage

Coupling: 15 minute coupling time recommended

Deprotection: RNA Deprotection-see bulletin Specifications

Diluent Anhydrous Acetonitrile/Dichloromethane 1:1 (v/v)

Recommended Storage Freezer storage, -10 to -30°C, dry Stability In Solution 1-2 Days

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200