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Oligo Synthesis

Oligo Synthesis : CEPs

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Pac-A-LA-CE Phosphoramidite

5'-Dimethoxytrityl-N-phenoxyacetyl-(2'-O, 4'-C methylene)-Adenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
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  • £
10-2601-02Pac-A-LA-CE Phosphoramidite0.25grams €350.75 Quantity Add to Order
10-2601-05Pac-A-LA-CE Phosphoramidite0.5grams €695.75 Quantity Add to Order

Description

Locked nucleic acid (LNA), or simply locked analog (LA), is a modified nucleic acid backbone consisting of a bicyclic sugar where the 2'-oxygen is connected to the 4'-carbon via a methylene unit. This additional ring restricts the sugar into a particularly favorable hybridization conformation. As a result, LA oligonucleotides exhibit unprecedented thermal stability towards complementary DNA and RNA.

Product Specifications

Formula: C49H54N7O9P
M.W.: 915.98
F.W.: 341.22
CAS Number: N/A

 

Dilution/Coupling Data
 

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of Additions

LV40LV20040nm0.2μm1μm10μm
10-2601-02 0.25 g 0.25 2.73 77.6 46.6 29.1 21.1 15.5 3.8
10-2601-05 0.5 g 0.5 5.46 168.6 101.2 63.2 46 33.7 8.4

Expedite

Catalog #Pack SizeGrams/PackDilution (mL)Approximate Number of Additions

Molarity50nm0.2μm1μm15μm
10-2601-02 0.25 g 0.25 4.07 0.07 75 46.8 34 4.6
10-2601-05 0.5 g 0.5 8.15 0.07 156.6 97.8 71.1 9.7

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References

Glen Report 37.2

SDS

Note that links go to manufacturer's website

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Applications & Benefits

Usage

Coupling: 3 minute coupling time recommended. Increase oxidation time 3 fold for optimal performance. To avoid any exchange of the iPr-Pac group on the G with acetyl, use the UltraMild Cap Mix A (40-4210-xx/40-4212-xx).

Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature OR 2 hours at Room Temperature in 30% Ammonium Hydroxide.

 

Specifications

Diluent: Anhydrous Acetonitrile

Recommended Storage: Freezer storage; -10 to -30°C, dry

Stability In Solution: 1-2 Days

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