Unit Definition: One unit is defined as the amount
of enzyme required to inhibit by 50% the hydrolysis of cytidine
2',3'-cyclic monophosphate by 5 ng of RNAse A.
Source: A recombinant E. coli strain carrying the porcine RNase Inhibitor gene.
| nit Concentration |
40,000 U/mL |
| Purity (SDS-PAGE) |
>99% |
| SS Exonuclease |
2000 U <5.0% released |
| DS Exonuclease |
2000 U <0.5% released |
| Endonuclease |
2000 U <10% converted |
| Non-specific RNAse |
2000 U none detected |
| E. coli 16S rDNA Contamination |
2000 U <10 copies |
| Storage |
-20 °C |
Supplied at 40,000 U/mL in 20 mM HEPES-KOH, 50 mM KCl, 8 mM
dithiothreitol, 50% glycerol, pH 7.5 @ 25°C.