RNase I
Product Description:
Ribonuclease I (RNase I) from E. coli degrades single-stranded
RNA cleaving after every base resulting in individual nucleoside
3'-monophosphates. RNase I does not require divalent cations for
activity and is not inhibited by ScriptGuard™ RNase Inhibitor, an RNase A-family inhibitor.
RNase I can be used for the following applications:
- Removal of RNA from DNA preparations.
- Mismatch detection in RNA:RNA and RNA:DNA hybrids.
- Degrades single-stranded RNA cleaving after every base.
- Reactions result in individual nucleoside 3'-monophosphates.
Product is for research use only (RUO)
Product Performance:
Figure 1. Ribosomal RNA is digested by RNase I. 30 μg of E. coli
ribosomal RNA was treated with varying amounts of RNase I for 5 min at
37°C. Complete degradation was achieved using 2 U of RNase I (lane 2).
Figure 2. RNase I removes RNA from a DNA:RNA solution. 30 μg of a plasmid prep from an E. coli culture containing a 16.3-kb recombinant clone was treated with
1 U of RNase I for 15 min at 37°C. RNA is degraded, leaving the plasmid DNA intact (lane 2).
| Components |
Volume |
RNase I, 10 U/μl
in 50% glycerol, 50 mM Tris-HCl, pH 7.5, 0.1 M NaCl, 0.1 mM EDTA and 0.1% Triton® X-100. |
100 μl |
10X RNase I Reaction Buffer
0.1 M Tris-HCl, pH 7.5, 1 M NaCl and 10 mM EDTA. |
1 ml |
Manual
SDS
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