ur PCR Quantification Standards contain genomic DNA extracted from
defined microorganisms, at low culture passage number. The DNA starting
material is analyzed with standardized methods to determine the DNA’s
integrity and quantity. Only pure and intact DNA showing a > 10 kb
band in an agarose gel and an OD260/280 > 1.8 is used for the
manufacture of these products. The DNA is partially sequenced to confirm
the species’ identity.
Technical Notes
Recommended Use
PCR Quantification Standards contain a defined number of genome
copies of a particular microorganism. Therefore, our standards are
perfectly suitable to generate DNA standard curves in qPCR. In addition,
standards are required for validating the specificity and testing the
sensitivity of PCR assays in the context of quality control and EP 2.6.7
compliant-testing.
Content
1 vial with 1x108 genomes (lyophilized)
3 vials with rehydration buffer (10 mM, pH 8.5)
Available Species and Ordering Information
- Cat. No. 52-0116 Acholeplasma laidlawii
- Cat. No. 52-1988 Aspergillus brasiliensis
- Cat. No. 52-0819 Aspergillus fumigatus
- Cat. No. 52-0347 Bacillus subtilis
- Cat. No. 52-1447 Bacteroides vulgatus
- Cat. No. 52-5571 Bordetella pertussis
- Cat. No. 52-1386 Candida albicans
- Cat. No. 52-1226 Candida glabrata
- Cat. No. 52-6128 Candida krusei
- Cat. No. 52-1953 Candida tropicalis
- Cat. No. 52-1664 Clostridium sporogenes
- Cat. No. 52-0083 Escherichia coli
- Cat. No. 52-0348 Kocuria rhizophila
- Cat. No. 52-0079 Lactobacillus acidophilus
- Cat. No. 52-0101 Legionella pneumophila
- Cat. No. 52-0129 Mycoplasma arginini
- Cat. No. 52-0117 Mycoplasma fermentans
- Cat. No. 52-0115 Mycoplasma gallisepticum
- Cat. No. 52-0130 Mycoplasma hyorhinis
- Cat. No. 52-0112 Mycoplasma orale
- Cat. No. 52-0119 Mycoplasma pneumoniae
- Cat. No. 52-0124 Mycoplasma synoviae
- Cat. No. 52-0103 Mycoplasma salivarium
- Cat. No. 52-0164 Spiroplasma citri
- Cat. No. 52-0848 Penicillium chrysogenum
- Cat. No. 52-0071 Pseudomonas aeruginosa
- Cat. No. 52-0799 Staphylococcus aureus
Shelf Life and Storage
The components must be stored between 2 and 8 °C (Shipping at ambient temperature).
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200