- Powerful lysis - Optimized bead beating method enables efficient lysis and solubilization of total nucleic acids and cellular proteins from fungi
- Easy and fast method - Novel spin filter-based method enables sequential DNA, RNA and protein extraction in less than 40 minutes without the need for precipitation
- Accurate results – Nucleic acids can be used in PCR, qPCR, RT-PCR, and next-generation sequencing; protein can be used for mass spectrometry, 1D and 2D gel electrophoresis
The AllPrep® Fungal DNA/RNA/Protein Kit is designed to isolate total nucleic acids and cellular proteins from fungal cultures in a user-friendly spin column format. An optimized bead beating method enables efficient lysis and solubilization of nucleic acids and proteins from diverse fungal species. The use of silica spin columns to achieve reversible, sequential immobilization of DNA, RNA and proteins greatly streamlines the nucleic acid and protein isolation process, enabling direct correlations between genes, their expression, and function. Isolated nucleic acids (gDNA, rRNA, mRNA, small RNAs) are suitable for the most demanding downstream applications including PCR, qPCR, RT-PCR, and next-generation sequencing (NGS). Purified proteins are suitable for 1D SDS-PAGE and mass spectroscopy following in-gel trypsin digestion. Some applications, including 2D SDS-PAGE and solution phase proteolytic digestion for mass spectrometry, may require an additional detergent removal step following protein elution.
This novel sequential isolation method begins with mechanical lysis of cultured fungal cells using a 0.5 mm glass bead tube. Nucleic acids and proteins are completely solubilized during homogenization and mixed with a DNA binding solution. DNA is bound to a spin column and the flow-through containing RNA and proteins is then combined with a solution that binds total RNA on a second spin column. The final flow-through, containing denatured proteins, is combined with another novel buffer to immobilize the proteins onto the third and final silica spin column. Each spin column containing either immobilized nucleic acids or proteins is then washed and the immobilized analyte is eluted.
For isolation of DNA, RNA and Protein from bacterial cultures, try our AllPrep® Bacterial DNA/RNA/Protein Kit.
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