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Oligo Synthesis

Oligo Synthesis : CEPs

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Pac-2-Amino-dA-CE Phosphoramidite

Pac-2-Amino-dA-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1585-02Pac-2-Amino-dA-CE Phosphoramidite0.25g £227.00 Quantity Add to Order
10-1585-90Pac-2-Amino-dA-CE Phosphoramidite100umole £114.00 Quantity Add to Order
10-1585-95Pac-2-Amino-dA-CE Phosphoramidite50umole £64.00 Quantity Add to Order

Description

Pac-2-Amino-dA-CE Phosphoramidite

5'-Dimethoxytrityl-N2-phenoxyacetyl-N6-di-n-butylaminomethylidene-2,6-diamino-2'-deoxypurine riboside-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C57H72N9O8P 
M.W.: 1042.21 
F.W.: 328.22

 

 
Description
The simplest approach to the design of high affinity primers and probes is to substitute A sites with 2-amino-A, since the 2-amino-A-T base pair is equivalent in strength to the G-T base pair. 2-Amino-A also destabilizes A-G wobble mismatches, thus increasing specificity. In 1998, we introduced a 2-amino-dA monomer which exhibits fast and effective deprotection in ammonium hydroxide and it is stabilized to depurination during synthesis. We now recommend the use of 0.5 M CSO in anhydrous acetonitrile (40-4632-xx) for best results with multiple additions of 2-amino-dA. This is because the bis formamidine protected 2-amino-dA leads to significant strand scission when standard iodine oxidation is used during synthesis. For this reason, we have also added Pac-2-Amino-dA, a monomer with optimized protection to meet the following criteria: stable during oligonucleotide synthesis, oxidation, and detritylation; labile towards common deprotection conditions (NH3, AMA, MeNH2); and the nucleobase protecting groups are cleaved under fairly mild conditions.
Technical documents
Safety Data Sheet (North America)
View Safety Data Sheet
Safety Data Sheet (EU)
View Safety Data Sheet
Safety Data Sheet (Other)
View Safety Data Sheet
Glen Report 27.23: New Product – Pac-2-Amino-dA
View Report
Glen Report 33-15: Technical Note - DNA and RNA Nucleoside Numbering System
View Report

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References

Glen Report 27.2: New Product - Pac-2'-Amino-dA

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Notes

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: 30% Ammonium Hydroxide for 4 hours at 65 °C or 8-17 hours at 55 °C. Compatible with 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) for 20 minutes at 65 °C.

Storage: Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 1-2 days

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200