- Remove 5’ cap structures (decapping) from various RNAs, including mRNA.
- Substitute unit-for-unit in any preexisting protocol using Tobacco Acid Pyrophosphatase (TAP).
Product is for research use only (RUO)
Product Description:
Cap-Clip™ Acid Pyrophosphatase is a plant-derived enzyme that
hydrolyzes various pyrophosphate bonds, including the pyrophosphate
bonds of the 5' cap of eukaryotic mRNAs, small nuclear RNAs (snRNAs),
heterogeneous nuclear RNAs (hnRNAs) and some viral RNAs. Complete
decapping generates RNA that has a 5'-monophosphate group.
Cap-Clip™ Acid Pyrophosphatase was developed as an improvement over
Tobacco Acid Pyrophosphatase (TAP). It can be used unit-for-unit in any
previously developed protocol that utilized TAP and has an identical
reaction buffer. With lot-to-lot consistency, absence of critical
contaminants and a dependable inventory, Cap-Clip™ Acid Pyrophosphatase
is a superior replacement for all TAP needs.
Materials Supplied:
Important Store at -20°C in a freezer without a defrost cycle. Do not store at -70°C.
|
Cap-Clip™ Acid Pyrophosphatase (100 units) |
|
Component |
Volume |
Cap-Clip™ Acid Pyrophosphatase, 5 U/μl
in 50% glycerol, 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM
dithiothreitol, 0.1 mM EDTA and 0.01% Triton® X-100 |
20 μl |
10X Cap-Clip™ Acid Pyrophosphatase Reaction Buffer
0.5 M NaOAc, pH 6.0, 10 mM EDTA, 1% β-mercaptoethanol and 0.1% Triton X-100 |
250 μl |
.
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