Cell Information:
Human umbilical vein smooth muscle cells (HUVSMC) are derived from the umbilical vein. Human umbilical vein actively regulates its vascular tone under the control of pO2. Vasodilation endothelial prostanoids contribute to the hypoxic vasodilation that counteracts the vasoconstricting actions of prostanoids released from HUVSMC. It was found that vasoconstricting prostanoids are released from the endothelium of the human umbilical vein at hyperoxia. During preeclampsia a total loss of neuronal nitric oxide synthase (nNOS) protein expression and a significant decrease in mRNA expression were seen. It was found that such loss might alter the regulation of blood flow in the fetal and maternal placental vasculature. Both ouabain and marinobufagenin activate proliferation of the HUVSMC. The concentration ranges of both compounds were found to be below that necessary to induce cytoplasmic ion alterations by sodium pump inhibition.
HUVSMC can be used as a powerful tool in studying blood vessel functions.
The Transfection Reagent:
Transfection of primary cells, such as HUVSMC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
Human Umbilical Vein Smooth Muscle Cell (HUVSMC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HUVSMC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HUVSMC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human umbilical vein smooth muscle cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human umbilical vein smooth muscle cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Umbilical Vein Smooth Muscle Cell Avalanche Transfection Reagent. |
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For Other Cells: |
Human Umbilical Vein Smooth Muscle Cell (HUVSMC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.
Human Prostate Smooth Muscle Cell (PrSMC)
Human Artery Smooth Muscle Cell
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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