Venor® Mycoplasma Extraction
The Venor® Mycoplasma Extraction kit is a DNA/RNA extraction kit designed to
separate nucleic acids from mycoplasma. It is
based on the extraction of DNA/RNA using magnetic beads. In mycoplasma
contamination control and detection, different samples such as cell
culture suspensions or cryostocks are analysed. The cell culture
supernatants to be tested contain different factors that can lead to
inhibition of PCR. Factors such as a large number of proteins in the
cell culture suspension or a to high confluency of more than 80-90% have
a major inhibiting influence on nucleic acid amplification techniques
(NAT). This is where the Venor® Mycoplasma Extraction kit comes in. The
Venor® Mycoplasma Extraction kit, with its fast performance and high extraction
efficiency, is adapted to our products of the Venor®GeM series, such as
the Venor®GeM qEP (Art.-No.: 11-9025) or also the Venor®GeM qOneStep
(Art.-No.: 11-91025), for the detection of mycoplasma.
Excellent reproducibility and quality due to automatic extraction
The Venor® Mycoplasma Extraction kit is also compatible with extraction
robots such as the KingFisher™ Flex. Its automated nucleic acid
extraction significantly improves the reproducibility of individual
extractions. Even when increasing the throughput by increasing the
number of samples up to 96 samples simultaneously, the DNA/RNA
extraction of the Venor® Mycoplasma Extraction kit maintains a constant quality.
The ready-to-use setup program for the KingFisher™ Flex can be
downloaded - see link in manual.
Recommended Use
For DNA/RNA extraction from:
- Cell culture supernatants
- Cell suspensions
- Cryostocks
- Biopharmaceuticals
Kit Components
- Binding, Elution, Lysis, Rehydration and Wash 1-3 Buffers
- Magnetic Bead Suspension
Proteinase K
Required Consumables
- 1-propanol ≥ 99,5% (required for the reconstitution of the Binding Buffer and the Wash Buffer 1 and 2)
- RNase-free 1.5 ml or 2 ml reaction tubes (preferably low-bind)
- Serological pipettes and pipettes with corresponding filter tips (100 and 1000 μl)
Required Lab Devices
For manual extraction:
- Magnetic rack and thermoshaker for 1.5 ml (or 2 ml) reaction tubes
- Optional: Microcentrifuge
For automatic extraction:
- Automatic extractor e.g. KingFisher™ Flex
- Plastic material (deep-well plates and elution plate)
Package Sizes
- Cat. No. 56-3010 10 extractions
- Cat. No. 56-3100 100 extractions
Storage
Ship and store at ambient temperature.
Attention: Remove the Proteinase K and store it at 4 °C upon receipt of the Venor® Mycoplasma Extraction kit.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200