E. cloni 10G Elite Electrocompetent Cells, Efficiency >2 x 1010 cfu/µg
E. cloni 10G Elite Electrocompetent Cells, 12 Reactions (SOLOs)
Clone, construct libraries, transform Gibson Assembly
® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging appli
cations.
Product information
E. cloni competent cells share the most useful genetic
elements of standard cloning strains like DH5α&trade,
DH10B&trade, JM109, TOP10, etc. and directly replace them in cloning
protocols. However, E. cloni electrocompetent cells
incorporate a unique manufacturing technology that increases
transformation efficiency, recombinant yields and reliability (Figures. 1
and 2), while decreasing costs. These cells provide solutions for a
wide range of applications at economical prices.
Choice of strain:
- E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.
- E. cloni 10GF′ Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.
Choice of efficiency:
- E. cloni 10G SUPREME Electrocompetent Cells > 4 × 1010 cfu/µg pUC DNA
SUPREME
Cells have the highest transformation efficiency available from any
supplier. Choose SUPREME Cells for the most demanding cloning
situations, such as construction of large, high complexity libraries or
cloning difficult targets, which require the greatest number of
transformants possible. - E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 × 1010 cfu/µg pUC DNA
ELITE
Cells have twice the transformation efficiency compared to “ultra high
efficiency” cells from other suppliers. ELITE Cells provide large
numbers of transformants from hard-to-clone fragments or limited DNA at a
lower price. - E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 109 cfu/µg pUC DNA
CLASSIC
Cells are high efficiency cells with a substantially lower cost per
reaction. These cells are the most economical choice for standard
cloning and library construction. 10G CLASSIC Cells are available in
larger package sizes for convenient use in higher volume cloning
applications.
Custom Competent Cells
Bulk cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.
Table 1. E. cloni Competent Cells for Cloning and Library Construction
E. cloni Cell Lines |
Transformation Efficiency (cfu/µg pUC DNA)
|
Cloning Methylated DNA
|
BAC, Cosmid Cloning
|
Blue/White Screening
|
10G SUPREME Electrocompetent
|
>4 × 1010
|
YES
|
YES
|
YES without IPTG induction
|
10G ELITE Electrocompetent
|
>2 × 1010
|
YES
|
NO
|
YES without IPTG induction
|
10G CLASSIC Electrocompetent
|
>5 × 109
|
YES
|
NO
|
YES without IPTG induction
|
10GF´ ELITE Electrocompetent
|
>2 × 1010
|
YES
|
NO
|
YES IPTG induction required |
SDS
Manuals and user guides
Product information sheets
F300 US ecloni comp cells cloning
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