E. cloni 10G Chemically Competent Cells
Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade
(12 transformations per tube), or microplates as indicated. Recovery
Medium is also available separately. The specified transformation
efficiencies are with pUC DNA, unless indicated otherwise.
Key features
- Direct replacements for standard cloning strains
- Optimised genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
- Available in a range of high transformation efficiencies
- Convenient packaging options.
Product information
E. cloni competent cells share the most useful genetic
elements of standard cloning strains like DH5α&trade,
DH10B&trade, JM109, TOP10, etc. and directly replace them in cloning
protocols. However, E. cloni electrocompetent cells
incorporate a unique manufacturing technology that increases
transformation efficiency, recombinant yields and reliability (Figures. 1
and 2), while decreasing costs. These cells provide solutions for a
wide range of applications at economical prices.
Choice of strain:
- E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.
- E. cloni 10GF′ Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.
Choice of efficiency:
- E. cloni 10G SUPREME Electrocompetent Cells > 4 × 1010 cfu/µg pUC DNA
SUPREME Cells have the highest transformation efficiency available from
any supplier. Choose SUPREME Cells for the most demanding cloning
situations, such as construction of large, high complexity libraries or
cloning difficult targets, which require the greatest number of
transformants possible. - E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 × 1010 cfu/µg pUC DNA
ELITE Cells have twice the transformation efficiency compared to “ultra
high efficiency” cells from other suppliers. ELITE Cells provide large
numbers of transformants from hard-to-clone fragments or limited DNA at a
lower price. - E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 109 cfu/µg pUC DNA
CLASSIC Cells are high efficiency cells with a substantially lower cost
per reaction. These cells are the most economical choice for standard
cloning and library construction. 10G CLASSIC Cells are available in
larger package sizes for convenient use in higher volume cloning
applications.
Plasmid cloning. E. cloni
10G SUPREME Cells provide recombinant yields higher than the highest
efficiency cells offered by a leading supplier (Figures 1 and 2)
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Figure 1. E. cloni 10G SUPREME
Electrocompetent Cells consistently outperformed “ultra high efficiency”
cells from a leading supplier. Both strains were transformed with 10 pg
of pUC19 (n=16).
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Figure 2.Transformation efficiency comparison of E. cloni
10G SUPREME Cells and Mega DH10B cells (Invitrogen). Competent cells
were transformed according to the manufacturer’s instructions with the
same pUC19 DNA control (provided with the Invitrogen cells)
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Table 1. E. cloni Competent Cells for Cloning and Library Construction
E. cloni Cell Lines |
Transformation Efficiency
(cfu/µg pUC DNA)
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Cloning Methylated DNA
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BAC, Cosmid Cloning
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Blue/White Screening
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10G SUPREME Electrocompetent
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>4 × 1010
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YES
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YES
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YES
without IPTG induction
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10G ELITE Electrocompetent
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>2 × 1010
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YES
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NO
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YES
without IPTG induction
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10G CLASSIC Electrocompetent
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>5 × 109
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YES
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NO
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YES
without IPTG induction
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10GF´ ELITE Electrocompetent
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>2 × 1010
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YES
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NO
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YES
IPTG induction required
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Genotypes
E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)
E. cloni 10GF´: [F´ pro A B lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)
Efficiencies
E. cloni 10G SUPREME Electrocompetent Cells > 4 × 1010 cfu/µg pUC DNA
E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 × 1010 cfu/µg pUC DNA
E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 109 cfu/µg pUC DNA
SDS
Manuals and user guides
Product information sheets
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