Cambio - Excellence in Molecular Biology

Competent Cells

Competent Cells: Competent cells

A range of electrocompetent and chemically competent cleverly designed E. coli strains for everyday cloning needs through to more demanding applications like CRISPR, working with unstable DNA or low copy number clones. 

HI-Control 10G Chemically Competent Cells

Stabilise toxic sequences behind T7 or lac-based promoters with this strain containing a LacI repressor, providing tight control over leaky expression

BioSearch Technologies (LGC)

Catalogue No.DescriptionPack SizePriceQty
60110-1HI-Control 10G Chemically Competent Cells, SOLOs) 12 reactions £196.00 Quantity Add to Order

Description

HI-Control 10G Chemically Competent Cells

 

Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

Key features

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  • Reduce leaky expression: The LacI repressor protein binds to the lacO operator, blocking transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter is greatly reduced.
  • Obtain high protein yields: Both 10G and BL21(DE3)-based strains express high levels of protein. For expression from a T7 promoter, select the HI-Control BL21(DE3) strain. Other lac-based promoters like T5-lac, tac, trc, and lac, can be used in either HI-Control strain.
  • Achieve high efficiency transformations: Highly efficient competent cells give excellent results from small amounts of plasmid DNA

 

Product information

The HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.

  Tightly Control Protein Expression    
  T7 Promoters Non-T7 lac promoters
(T5-lac, tac, trc, lac)		 Clone with low background target expression Transformation efficiencies
HI-Control
BL21(DE3)		 X X   ≥ 1×107 cfu/µg
HI-Control
10G 		  X X ≥ 1×109 cfu/µg

Genotypes:

HI-Control BL21 (DE3): F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)

HI-Control 10G: mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ?80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA Mini-F lacIq1 (GentR)

 

Please note that the SUMO Protease and SUMO Fusion tag are modified from the original native proteins. Native or non-modified control proteins or proteases will not be compatible.

SDS

Manuals and user guides

HI Control

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200