E. cloni EXPRESS Competent Cells
Each E. cloni EXPRESS Kit contains: the indicated E. cloni
EXPRESS Electrocompetent or Chemically Competent Cells in DUO
packaging (2 transformations per tube), Expression Recovery Medium
(lactose minus), pUC19 Positive Control Plasmid, and complete protocols.
Product information
E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the
first to offer high efficiency cloning and high level protein expression
in the same cell. Cloning efficiencies are increased 25-1,000 fold
relative to competitors’ preparations of BL21 cells (Figure 1), which is
essential for construction of complex expression libraries. The
unprecedented transformation efficiency of E. cloni EXPRESS Electrocompetent Cells (> 5 × 109
cfu/µg) eliminates the need for plasmid transfer from the cloning
strain to the expression strain, saving days of work in a typical
cloning and expression experiment (Figure. 2).
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Figure 1. Transformation efficiency comparison of commercially available BL21(DE3) competent cells.
Figure 2. Comparison of cloning and protein expression procedures.
E. cloni EXPRESS BL21(DE3) strains contain the phage T7 RNA
polymerase gene linked to the IPTG-inducible promoter, for use with any
expression plasmid containing the T7 promoter.
NOTE: For best results in expressing proteins that may be toxic, we recommend using OverExpress Cells. E. cloni EXPRESS cells are also available as chemically competent cells. With a transformation efficiency of >1 × 107
cfu/µg and very economical prices, EXPRESS Chemically Competent Cells
offer superior value for everyday protein expression work.
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- Best value for routine protein expression
- Available as Electrocompetent and Chemically Competent Cells.
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Genotype
E. cloni EXPRESS BL21(DE3): F– ompT hsdSB (rB- mB-) gal dcm lon λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
SDS
Manuals and user guides
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200