TG1 Electrocompetent Cells
Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs
(2 trans
formations per tube). Recovery Medium is also available
separately. The specified transformation efficiencies are with pUC DNA,
unless indicated otherwise.
TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010
cfu/µg) electrocompetent cells for phage display library screening.
These cells are used for phage display and protein expression
Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.
Table 1. Competent Cells for Phage Display libraries
Cell Lines |
Transformation Efficiency
(cfu/µg pUC DNA)
|
Cloning Methylated DNA
|
BAC, Cosmid Cloning
|
Blue/White Screening
|
|
TG1 Electrocompetent
|
>4 × 1010
|
YES
|
NO
|
YES
IPTG induction required
|
|
SS320 (MC1061 F') Electrocompetent
|
>4× 1010
|
YES
|
NO
|
YES
IPTG induction required
|
|
MC1061 F-Electrocompetent
|
>4× 1010
|
YES
|
NO
|
YES
IPTG induction required
|
|
ER2738 Electrocompetent
|
>2 × 1010
|
YES
|
NO
|
YES
IPTG induction required
|
Genotype
- TG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)
- SS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK ) mcrA mcrB1
- ER2738: [F'proA B lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
- MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK ) mcrA mcrB1
Product information sheets
F302 US0218 phage display comp cells
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