Cambio - Excellence in Molecular Biology

Competent Cells

Competent Cells: Competent cells

A range of electrocompetent and chemically competent cleverly designed E. coli strains for everyday cloning needs through to more demanding applications like CRISPR, working with unstable DNA or low copy number clones. 

SS320 (MC1061 F') Electrocompetent Cells

Construct larger, more complex phage display libraries with highest efficiency cells. SS320 is a non-amber suppressor strain used for phage display.

BioSearch Technologies (LGC)

Catalogue No.DescriptionPack SizePriceQty
60512-1SS320 (MC1061 F') Electrocompetent Cells, (DUOs) 12 reactions £233.00 Quantity Add to Order
60512-2SS320 (MC1061 F') Electrocompetent Cells, (DUOs) 24 reactions £385.00 Quantity Add to Order

Description

SS320 (MC1061 F') Electrocompetent Cells

Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

Construct larger, more complex phage display libraries with highest efficiency cells. SS320 is a non-amber suppressor strain used for phage display.

 

 

Product information

SS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.

 

Key features

  • Perfect for antibody phage display library creation
  • Create larger libraries; speed discovery
  • Bulk custom dispensing available with all of our phage display cells

 

 

Transformation efficiency of Biosearch Technologies electrocompetent bacterial cells for phage display compared to competitors

Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.

Table 1. Competent Cells for Phage Display libraries

 


Cell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

TG1 Electrocompetent

>4 × 1010

YES

NO

YES
IPTG induction required

SS320 (MC1061 F') Electrocompetent

>4× 1010

YES

NO

YES
IPTG induction required

MC1061 F-Electrocompetent

>4× 1010

YES

NO

YES
IPTG induction required

ER2738 Electrocompetent

>2 × 1010

YES

NO

YES
IPTG induction required

 

Genotype

 

  • TG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)
  • SS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK ) mcrA mcrB1
  • ER2738: [F'proA B lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
  • MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK ) mcrA mcrB1

 

SDS

Manuals and user guides

Product information sheets

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