E. cloni 5-alpha Chemically Competent Cells
Competent Cells include Control DNA and Recovery Medium, and are
packaged as DUOs (2 transformations per tube). Recovery Medium is also
available separately. The specified transformation efficiencies are with
pUC DNA, unless indicated otherwise
- Direct replacements for standard cloning strains
- Optimised genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.
E. cloni competent cells share the most useful genetic elements of
standard cloning strains like DH5α™ and directly replace them in cloning
protocols. E. cloni chemically competent cells provide the performance
researchers need with ease of use—only common laboratory equipment is
required. These cells provide solutions for a wide range of applications
at economical prices.
E. cloni 5-alpha Chemically Competent Cells >1 × 10 8 cfu/µg DNA
Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
Table 1. E. cloni Competent Cells for Cloning
E. cloni Cell Lines |
Transformation Efficiency
(cfu/µg pUC DNA)
|
Cloning Methylated DNA
|
BAC, Cosmid Cloning
|
Blue/White Screening
|
|
5-alpha Chemically Competent
|
>1 × 10 8 cfu/µg DNA
|
NO
|
NO
|
YES
No IPTG induction required
|
E. cloni 5-alpha: fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
Efficiencies
E. cloni 5-alpha Chemically Competent Cells >1 × 108 cfu/µg DNA
Manuals and user guides
E cloni alpha Chemically Competent Cells
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