RNase R

Ribonuclease R (RNase R) from E. coli, is a 3′-5′ exoribonuclease that can digest linear RNA and Y-structure
RNA while leaving circular RNA (circRNA) and lariat intron RNA intact. RNase R is a crucial component of
circRNA clean up and is ideal for enriching circRNA in total cellular RNA preparations or removing linear RNA
from in vitro circRNA preparations using RNA Ligase-based and self-splicing permutated intron-exon sequence
(PIE method)-based methodologies.

Benefits:
• Efficient circRNA clean up: Ideal for enriching circRNA in total RNA preparations.
• Targeted digestion: Digests linear RNA and Y-structure RNA, leaving circRNA and lariat intron RNA intact.
• Fast workflow: 1 μg of RNase R digests 5 μg of linear RNA in just 30 minutes.
• No protocol guesswork: Full product manual with troubleshooting tips included.

Product Description
Ribonuclease R (RNase R) from E. coli, is a 3′?5′ exoribonuclease that can digest linear RNA and Y-structure
RNA but does not digest circular RNA (circRNA), lariat intron RNA, blunt-ended double-stranded RNA (dsRNA),
dsRNA with 3′-overhangs <7 nt in length or single-stranded DNA. Highly structured linear cellular RNAs such
as prokaryotic 23S and 16S ribosomal RNAs (rRNAs), small nuclear RNAs (snRNAs), transfer RNAs (tRNAs)
and histone mRNAs are also not efficiently degraded by RNase R. This functionality makes RNase R ideal for
enriching circRNA structures from total cellular RNA preparations or in vitro circRNA preparations. 1 μg of RNase
R will digest 5 μg of linear RNA in 30 minutes at 37°C.

Figure 1. A PIE-method RNA circularization preparation was assessed on a denaturing PAGE gel before
(UT= Untreated) and after (T= Treated) RNase R treatment. The untreated sample lane shows bands of various
sizes corresponding to different length linear RNA subproducts from the PIE-method. Following RNase R
treatment, the linear RNAs are digested, leaving only the circular RNA (red arrow) intact.

Figure 2. 5 μg of linear RNA was circularized with RNA Ligase to produce circular RNA (lane 2). The RNA ligation
reaction product was treated with 1 μg of RNase R at 37°C for 30 minutes to enrich for circular RNAs (lane 3).

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• Manual
• SDS

These are both links to the manufacturer's page

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

1. Suzuki, H. et al., (2006) Nucl. Acids Res. 34 (8) e63.

2. Vincent, H.A. and Deutscher, M.P., (2006) J. Biol. Chem. 281 29769.

3. Xiao, M.S. and Wilusz, J.E., (2019) Nucl. Acids Res. 47 8755.

4. Wesselhoeft, R.A. et al., (2018) Nat Commun. 9 2629.

5. Qui, Z. et al., (2022) BioRxiv. doi: 10.1101/2022.06.20.496777.

6. Beaudry, D. and Perreault, J.P., (1995) Nucl. Acids Res. 23 3064.

7. Puttaraju, M. and Been, M.D., (1996) J. Biol. Chem. 271 26081

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

• Digests linear RNA and Y-structure RNA, leaving circRNA, lariat intron RNA, and dsRNA intact.
• Ideal for isolating circRNA from total RNA preparations.
• 1 µg of RNase R digests 5 µg of linear RNA in 30 minutes.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200