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Oligo Synthesis

Oligo Synthesis : CEPs

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Universal-CE Phosphoramidite

3-[(3aR,4R, 5R, 6S, 7S)-6-(4,4'-dimethoxytrityloxy)-5-acetoxy-1-,3-dioxooctahydro-1H-4,7-epoxyisoindol-2-yl]propyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-5000-02Universal-CE Phosphoramidite0.25g £91.00 Quantity Add to Order
10-5000-90Universal-CE Phosphoramidite100µmoles £41.00 Quantity Add to Order

Description

Cleavable linkers have a wide range of application uses. We now offer Universal-CE, a dual-purpose linker capable of revealing 3’-OH termini or serving as a 5’-amino modifier C3 upon cleavage. Used as a universal phosphoramidite, the resulting oligonucleotides with a 3’-OH may be crucial for downstream applications, such as primer extension or ligation assays. Used as an amino modifier, the resulting oligonucleotides would be identical to those generated with 5’-Amino-Modifier C3-TFA.

Product Specifications

Formula: C43H52N3O10P
M.W.: 801.87
CAS Number: 730963-37-8

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Deprotect using Ammonium Hydroxide:MethylAmine (AMA) 1:1 for 1 hour at 65 °C or Ammonium Hydroxide for 8 hours at 55 °C. For sensitive minor bases or dyes, use 50 mM Potassium Carbonate in Methanol in 17 hours at room temperature or with Tert-Butylamine/water 1:3 (v/v) for 4 hours at 60 °C.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions

LV40 LV200 40nm 0.2μm 1μm 10μm
10-5000-90 100 µmol 0.08 1 20 12 7.5 5.45 4 1
10-5000-02 0.25 g 0.25 3.11 90.33 54.2 33.98 24.64 18.07 4.52

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions

Molarity 50nm 0.2μm 1μm 15μm
10-5000-90 100 µmol 0.08 1.5 0.07 23.6 14.75 10.73 1.48
10-5000-020.25 g0.254.650.0786.654.1339.365.41

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References

References

The Glen Report, 2021, 33.1, 6-8.

The Glen Report, 2003, 16.2, 8-9. The Glen Report, 2011, 23.1, 10-11. W.

Zhou, G. McGall, and V. Singh: February 2017, United States Patent, Centrillion Technology Holdings Corporation, PCT/US2016/047488.

The Glen Report, 2013, 25.1, 11-12.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

Safety Data Sheet

Glen Report 34-17: New Product — Universal-CE Phosphoramidite

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

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