Catalog Number: 20-3304-xx
(20-3404-xx)
Description: Ac-A-RNA-CPG
5'-Dimethoxytrityl-N-acetyl-Adenosine,2'-O-acetyl-3'-succinoyl-
long chain alkylamino-CPG |
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F.W.: 329.21 |
Diluent: Not Applicable |
| Coupling: Please refer to the technical bulletin for the synthesis and deprotection procedures. This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. |
| Deprotection: Deprotect using the protocol required by the nucleobases. Technical Bulletin |
| Storage: Controlled room temperature or lower, dry |
| Stability in Solution: Not Applicable |
TOM-Protected RNA Phosphoramidites
RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2’-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2’ to 3’ migration. This migration under basic conditions leads to non-biologically active 2’-5’ linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2’-O-TBDMS protection.
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