Cambio - Excellence in Molecular Biology

Extraction & Purification - RNA

Extraction & Purification - RNA: Microbial

Specialised RNA purification kits for the isolation of a variety of microbial samples, including yeast.

MasterPure™ Yeast RNA Purification Kit

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from cell types including: Candida, Saccharomyces, Schizosaccharomyces, and filamentous fungi

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
MPY03100MasterPure™ Yeast RNA Purification Kit100 reactions £262.00£262.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

MasterPure™ Yeast RNA Purification Kit

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from cell types including: Candida, Saccharomyces, Schizosaccharomyces, and filamentous fungi

BioSearch Tech (Lucigen/Epicentre)

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from cell types including: Candida, Saccharomyces, Schizosaccharomyces, and filamentous fungi.
The kit utilizes a rapid desalting process to remove contaminating macromolecules, avoiding toxic organic solvents, bead-beating, and spheroplasting.

S. cerevisiae RNA
Labelled cDNA from MasterPure RNA Figure 1. RNA purified with the MasterPure™ Yeast RNA Purification Kit was reverse-transcribed, labelled, and hybridized to an S. cerevisiae DNA microarray. The cDNA from cells under oxidative stress was labelled with Cy5, and cDNA from control cells was labelled with Cy3.
S. cerevisiae RNA Labeled cDNA from MasterPure RNA

 

Figure 2. Agilent Technologies 2100 Bioanalyzer electrophoretogram of the S. cerevisiae RNA shown in Figure 1. Figure 2. Agilent Technologies 2100 Bioanalyzer© electrophoretogram of the S. cerevisiae RNA shown in Figure 1. RNA was purified by the MasterPure™ Yeast RNA Purification Kit and stored at -20° C for 2 months before analysis. Observe the high purity, intact RNA.

 




If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MasterPure™ Yeast RNA Purification Kit

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from cell types including: Candida, Saccharomyces, Schizosaccharomyces, and filamentous fungi

BioSearch Tech (Lucigen/Epicentre)

Protocols for: MasterPure™ Yeast RNA Purification Kit

MasterPure™ Yeast RNA Purification Kit Protocol

(catalogue number MPY03010 / MPY03100)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MasterPure™ Yeast RNA Purification Kit

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from cell types including: Candida, Saccharomyces, Schizosaccharomyces, and filamentous fungi

BioSearch Tech (Lucigen/Epicentre)

References 

  1. Miller, S.A. et al., (1988) Nucl. Acids Res . 16, 1215.
  2. Hoffman, L. and B.W. Jarvis. (2004) EPICENTRE Forum 11 (2), 4.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MasterPure™ Yeast RNA Purification Kit

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from cell types including: Candida, Saccharomyces, Schizosaccharomyces, and filamentous fungi

BioSearch Tech (Lucigen/Epicentre)

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MasterPure™ Yeast RNA Purification Kit

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from cell types including: Candida, Saccharomyces, Schizosaccharomyces, and filamentous fungi

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Purification of RNA from yeast and filamentous fungi suitable for cDNA synthesis and microarray gene expression analysis.

Benefits

  • Avoids hot acid phenol.
  • Faster than spheroplasting.
  • Higher quality RNA than bead-beating.
  • No extra enzymes or equipment to purchase.
  • Yields 25-50 µg RNA from 1 ml of mid-log S.cerevisiae.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200