Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : Purification

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.


Poly-Pak Cartridges

Poly-Pak Cartridges

Glen Research

Catalogue No.DescriptionPack SizePriceQty
60-1100-01Poly-Pak Cartridges Pk/1 £9.00 Quantity Add to Order
60-1100-10Poly-Pak Cartridges Pk/10 £73.00 Quantity Add to Order

Description

Poly-Pak Cartridges

Catalog Number: 60-1100-xx

Description: Poly-Pak™ Cartridges



Coupling:
Deprotection: Technical Bulletin
Storage: Controlled room temperature or lower, dry

Stability in Solution: No data available

POLY-PAK™ PURIFICATION

The use of Poly-Pak™ packings in cartridges or barrels overcomes several disadvantages usually associated with reverse phase (RP) cartridges. The packing is stable in the pH range 1-13, thus the ammonium hydroxide solution, diluted with water, is loaded directly onto the packing. Also, after elution of failure sequences, the trityl group is removed and washed from the support-bound oligonucleotide. The fully deprotected product can then be eluted and isolated by lyophilization. Poly-Pak™ Cartridges may also be used for desalting normal or labelled oligonucleotides. The original Poly-Pak cartridge and barrel are designed for 0.2 µmole syntheses or less. Poly-Pak II cartridges and barrels are designed for use with 1 µmole syntheses. A booklet, User Guide To Poly-Pak™ Cartridge Purification, describes in detail the process and several applications. This booklet is delivered with cartridges, barrels, and packing and is available on request.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

Material Safety Data Sheet

Glen Report 8.2: 5' LABELLING - FLUORESCEIN AND CYANINE DYES, BIOTIN

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
60-1100-01 Pk/1 1grams N/A N/A N/A N/A N/A N/A N/A
60-1100-10 Pk/10 1grams N/A N/A N/A N/A N/A N/A N/A
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
60-1100-01 Pk/1 1grams N/A N/A N/A N/A N/A
60-1100-10 Pk/10 1grams N/A N/A N/A N/A N/A
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
60-1100-01 Pk/1 1grams N/A N/A N/A N/A
60-1100-10 Pk/10 1grams N/A N/A N/A N/A

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200