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Oligo Synthesis

Oligo Synthesis : CEPs

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Solid Chemical Phosphorylation Reagent II

Solid Chemical Phosphorylation Reagent II

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1902-02Solid Chemical Phosphorylation Reagent II 0.25g £182.00 Quantity Add to Order
10-1902-90Solid Chemical Phosphorylation Reagent II 100µmoles £55.00 Quantity Add to Order

Description

Solid Chemical Phosphorylation Reagent II

 

Structure

 

Catalog Number: 10-1902-xx

Description: Solid Chemical Phosphorylation Reagent II

[3-(4,4'-Dimethoxytrityloxy)-2,2-dicarboxymethylamido]propyl-
(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Formula: C37H49N4O7P M.W.: 692.79 F.W.: 79.98
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: If the final DMT is removed during synthesis, deprotection under standard conditions will eliminate the Solid CPR II to yield a 5' Phosphate. If the DMT is retained, see Technical Bulletin for removal procedure (Technical Bulletin).
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: No data available
Please Note: Chemical Phosphorylation Reagent II is covered by US Patent No.: 5,959,090 and European Patent: EP0816368.

CHEMICAL PHOSPHORYLATION

Chemical Phosphorylation Reagent is most commonly used to phosphorylate the 5'-terminus of an oligonucleotide. Although this product is also successful in 3'-phosphorylation, 3'-Phosphate CPG allows direct preparation of oligonucleotides with a 3'-phosphate group. Chemical Phosphorylation Reagent II contains a DMT group on a side chain which is stable to base cleavage and can be left on the oligonucleotide for use in RP purification. The DMT group is later removed with aqueous acid and the side chain is eliminated after brief treatment with aqueous ammonium hydroxide to yield the 5'-phosphate.1 Solid CPR II is similar in performance to CPR II but it is easier to prepare aliquots since it is a powder. Many researchers treat synthesis supports with a hindered base (e.g., diethylamine, diisopropylethylamine, or DBU) post-synthesis to eliminate and remove the cyanoethyl phosphate groups. In this way, the acrylonitrile formed in situ is removed from the support and is not available to alkylate dT residues at the N3 position in the oligos. Since the sulfonylethyl group in 3'-Phosphate CPG is also susceptible to ß-elimination leading to oligo cleavage, this technique is not compatible with 3'-phosphate CPG. Using CPR II CPG, which is base labile but does not support ß-elimination, the cyanoethyl groups can be removed from the oligo prior to cleavage and base deprotection. ABI-style vials and columns are supplied unless otherwise requested.

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Protocols

Material Safety Data Sheet

Glen Report 20.1: New Products

Glen Report 23.1: Technical Brief - Chemical Phosphorylation ? considering the options

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References

Chemical Phosphorylation Reagent is provided under license from Siemens Corporation.

Chemical Phosphorylation Reagent II is covered by US Patent No.: 5,959,090.

(1) A. Guzaev, H.Salo, A. Azhayev, and H. Lonnberg, Tetrahedron, 1995, 51, 9375-9384.

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Notes

 Solid Chemical Phosphorylation Reagent II

[3-(4,4'-Dimethoxytrityloxy)-2,2-dicarboxymethylamido]propyl-
(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Formula: C37H49N4O7P M.W.: 692.79 F.W.: 79.98
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: If the final DMT is removed during synthesis, deprotection under standard conditions will eliminate the Solid CPR II to yield a 5' Phosphate. If the DMT is retained, see technical bulletin for removal procedure. Technical Bulletin
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: No data available
Please Note: This product is covered by US Patent No.: 5,959,090.
Material Safety Data Sheet

Literature Highlights

Glen Report 20.1: New Products

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1902-90 100µmoles .069grams 1 20 12 7.5 5.45 4 1
10-1902-02 0.25grams .25grams 3.61 107 64.2 40.13 29.18 21.4 5.35
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1902-90 100µmoles .069grams 1.5 .067 23.6 14.75 10.73 1.48  
10-1902-02 0.25grams .25grams 5.39 .067 101.4 63.38 46.09 6.34  
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1902-90 100µmoles .069grams 1.5 .067 25.2 15.75 11.45    
10-1902-02 0.25grams .25grams 5.39 .067 103 64.38 46.82

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1902-90 100µmoles .069grams 1 20 12 7.5 5.45 4 1
10-1902-02 0.25grams .25grams 3.61 107 64.2 40.13 29.18 21.4 5.35
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1902-90 100µmoles .069grams 1.5 .07 23.6 14.75 10.73 1.48
10-1902-02 0.25grams .25grams 5.39 .07 101.4 63.38 46.09 6.34
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1902-90 100µmoles .069grams 1.5 .07 25.2 15.75 11.45

10-1902-02 0.25grams .25grams 5.39 .07 103 64.38 46.82

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200