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Oligo Synthesis

Oligo Synthesis : CEPs

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Universal Tosyl Phosphoramidite

Universal Tosyl Phosphoramidite

Glen Research

Description

 

10-1929
 

Catalog Number: 10-1929-xx

Description: Universal Tosyl Phosphoramidite

THIS ITEM HAS BEEN DISCONTINUED
3-(4,4'-dimethoxytrityloxy)-1-toluenesulfonyl-propyl-
2-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Formula: C40H49N2O8PS M.W.: 748.86 F.W.: variable
Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/ProductFiles/Technical/TB_ABITransfer.pdf.
 
 
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

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Protocols

Material Safety Data Sheet

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?Glen Report 22.2: New Product - Universal Tosyl Phosphoramidite

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References

  1. P A Bertin, et al., J Amer Chem Soc., 2006, 128, 4168-9
  2. M K Herrlein, J S Nelson, and R L Letsinger, J Amer Chem Soc., 1995, 117, 10151-10152
  3. R L Letsinger, T F Wu, and R Elghanian, Nucleosides and Nucleotides, 1997, 16, 643-652
  4. C S Thaxton, et al., Proc Natl Acad Sci USA, 2009, 106, 18437-42

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1929-02 0.25grams .25grams 3.34 98 58.8 36.75 26.73 19.6 4.9
10-1929-90 100µmoles .075grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1929-02 0.25grams .25grams 4.98 .07 93.2 58.25 42.36 5.83  
10-1929-90 100µmoles .075grams 1.5 .07 23.6 14.75 10.73 1.48  
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1929-02 0.25grams .25grams 4.98 .07 94.8 59.25 43.09    
10-1929-90 100µmoles .075grams 1.5 .07 25.2 15.75 11.45  

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