Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Smart Buffers and Reagents

Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions.

Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago's SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures.

A high level of automation essentially avoids operator intervention and contact with the products.

SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches.

•Pre-mixed powder buffers or tablets with pre-set pH

•Analytical grade reagents

•Eliminates calculation, formulation and weighing errors

•Dissolve-and-go for greater convenience

•Stable at room temperature for at least 3 years

•Guaranteed reproducibility


Glycine-HCl buffer 0.1 M pH 3.0 1000 ml

Medicago’s Glycine-HCl buffer can be used for reactions when pH must be maintained at 3.0. The buffer can be used as elution buffer in affinity chromatogaphy (1) for example antibodies from Protein A (2) and lectins.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
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MC-12-9121-10Glycine-HCl buffer 0.1 M pH 3.0 1000 ml 10 pouches €210.00 Quantity Add to Order

Description

 Product description

Medicago’s Glycine-HCl buffer can be used for reactions when pH must be maintained at 3.0. The buffer can be used as elution buffer in affinity chromatogaphy (1) for example antibodies from Protein A (2) and lectins.

Glycine-HCl is supplied as a powder mix in pouches giving 1000 ml of 0.1 M glycine-HCl with pH 3.0 at 25°C when dissolved in deionized water.

Directions for use

Empty one pouch of the glycine-HCl buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 1000 ml, stir until full dissolution and the buffer solution is ready to use.

 

Shipping and storage

Glycine-HCl buffer is shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.

Specifications

Chemicals: Analytical grade

Format: Exactly pre-weighed powder mix

Concentration: 0.1 M glycine-HCl

Volume: 1000 ml

pH: 3.0 ± 0.05 at 25°C

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

click here to download the protocol

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

Features

  • Formulated from analytical grade reagents
  • Pre-set pH
  • Exactly pre-weighed in pouches
  • Dissolve and use in minutes
  • Ideal for standardizing laboratory work

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

Applications

  • Elution buffer for chromatography procedures

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200