Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Smart Buffers and Reagents

Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions.

Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago's SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures.

A high level of automation essentially avoids operator intervention and contact with the products.

SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches.

•Pre-mixed powder buffers or tablets with pre-set pH

•Analytical grade reagents

•Eliminates calculation, formulation and weighing errors

•Dissolve-and-go for greater convenience

•Stable at room temperature for at least 3 years

•Guaranteed reproducibility


Sodium Phosphate Buffer (NaPi)

Medicago’s Sodium Phosphate buffer is provided in different formulations in order to suit most biochemical and molecular biology applications. Choose a buffer with a pH and molarity that fits your laboratory procedures.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-12-9184-10Sodium Phosphate Buffer (NaPi) 1M pH 6.5, 1000 ml 10 pouches £283.00 Quantity Add to Order
MC-12-9185-10Sodium Phosphate Buffer (NaPi) 1M pH 7.2, 1000 ml 10 pouches £283.00 Quantity Add to Order
MC-12-9527-10Sodium Phosphate Buffer (NaPi) 0.02M pH 7.0, 1000 ml 10 pouches £168.00 Quantity Add to Order
MC-12-9528-10Sodium Phosphate Buffer (NaPi) 0.02M pH 7.0, 5000 ml 10 pouches £295.00 Quantity Add to Order
MC-12-9529-10Sodium phosphate buffer (NaPi) 0.1M pH 6.5, 1000 ml 10 pouches £185.00 Quantity Add to Order
MC-12-9530-10Sodium phosphate buffer (NaPi) 0.1M pH 7.0, 1000 ml 10 pouches £185.00 Quantity Add to Order
MC-12-9531-10Sodium phosphate buffer (NaPi) 0.1M pH 7.4, 1000 ml 10 pouches £185.00 Quantity Add to Order
MC-12-9532-10Sodium phosphate buffer (NaPi) 0.1M pH 6.0, 1000 ml 10 pouches £185.00 Quantity Add to Order
MC-12-9533-10Sodium phosphate buffer (NaPi) 0.1M pH 6.8, 1000 ml 10 pouches £185.00 Quantity Add to Order
MC-12-9534-10Sodium phosphate buffer (NaPi) 0.1M pH 7.2, 1000 ml 10 pouches £185.00 Quantity Add to Order
MC-12-9535-10Sodium phosphate buffer (NaPi) 0.1M pH 7.5, 1000 ml 10 pouches £185.00 Quantity Add to Order
MC-12-9536-10Sodium phosphate buffer (NaPi) 0.1M pH 8.0, 1000 ml 10 pouches £185.00 Quantity Add to Order

Description

Product description

Medicago’s Sodium Phosphate buffer is provided in different formulations in order to suit most biochemical and molecular biology applications. Choose a buffer with a pH and molarity that fits your laboratory procedures.

The NaPi buffer is supplied as exactly pre-weighed powder in sealed pouches giving two volumes, 1000 ml and 5000 ml.
Dissolving the contents of one pouch in deionized water yields:

1) NaPi buffer 0.1 M or 1 M with pH 6.5 at 25°C.

2) NaPi buffer 0.1 M or 0.02 M with pH 7.0 at 25°C.

3) NaPi buffer 1 M with pH 7.2 at 25°C.

4) NaPi buffer 0.1 M or 1 M with pH 7.4 at 25°C.

 Applications

  • Multi-purpose buffer for many laboratory procedures

 

Directions for use

Empty one pouch of the NaPi buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 200 ml to 300 ml of deionized water for a 1000 ml pouch and 1000 ml for a 5000 ml pouch and stir the solution for a few minutes. Adjust the volume up to 1000 ml or 5000 ml, stir and the buffer solution is ready to use.

 

Shipping and storage

NaPi buffer is shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

Specifications

Chemicals: Analytical grade

Format: Exactly pre-weighed powder mix

Concentration: 1. 0.1 M or 1 M, pH 6.5 at 25°C

                      2. 0.1 M or 0.02 M, pH 7.0 at 25°C

                      3. 1 M, pH 7.2 at 25°C

                      4. 0.1 M or 1 M, pH 7.4 at 25°C

Volume: 1000 ml and 5000 ml

pH:   6.5 ± 0.05 at 25°C
        7.0 ± 0.05 at 25°C
        7.2 ± 0.05 at 25°C
        7.4 ± 0.05 at 25°C

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

Features

  • Purity of chemicals minimum 99%
  • Choice of three molar concentrations
  • Wide choice of pH
  • Exactly pre-weighed in pouches
  • Dissolve and use in minutes

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200