Applications

Oligo Synthesis : CEPs

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tCnitro-CE Phosphoramidite

Glen Research

Catalogue No.	Description	Pack Size	Price	Qty	Change to: £
10-1518-02	tCnitro-CE Phosphoramidite	0.25g	€2,018.40	Quantity	Add to Order
10-1518-90	tCnitro-CE Phosphoramidite	100umole	€709.20	Quantity	Add to Order
10-1518-95	tCnitro-CE Phosphoramidite	50umole	€354.00	Quantity	Add to Order

Description

structure 10-1518

Catalog Number: 10-1518-xx

Description: tC_nitro-CE Phosphoramidite

5'-O-(4,4'-Dimethoxytrityl)-1'-(7-nitro-1,3-diaza-2-oxophenothiazin-1-yl)- 2'-deoxy-Β-D-ribofuranosyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C₄₅H₄₉N₆O₉PS	M.W.: 880.94	F.W.: 440.32

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: As required by nucleobases. Compatible with Ammonium Hydroxide for 17 hours at room temperature, Ammonium hydroxide/40% methylamine 1:1 (AMA) for 10 minutes at 65°C or UltraMild deprotection conditions.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

Please Note: These products are offered under license from ISIS Pharmaceuticals, Inc. and in collaboration with ModyBase HB.

The tricyclic fluorescent nucleoside analogues, 1,3-diaza-2-oxophenothiazine, tC, and 1,3-diaza-2-oxophenoxazine, tCo, are deoxycytidine analogs that have been shown to base pair faithfully with dG with virtually no disruption of the normal duplex structure. This means that the stability of the DNA duplex is not compromised as compared to the control regardless of DNA sequence. The fluorescence quantum yield of tC is essentially unchanged between single stranded and double stranded DNA - 0.21 for single stranded DNA and 0.19 for duplex DNA. Also, the fluorescence characteristics of tC are not sensitive to neighboring base combinations. tCo has been shown to be the brightest fluorescent nucleoside analogue in duplex context reported so far and even retains the majority of its fluorescence when surrounded by guanine residues. Indeed, tCo has been reported to be 25-50 times brighter than 2-aminopurine.