Cambio - Excellence in Molecular Biology

Cell Culture Contamination Control

Cell Culture Contamination Control: Elimination

Mycoplasma Elimination

 
If you are unfortunate enough to find mycoplasma in your cell culture or virus stocks we highly recommend immediate autoclaving and disposal of any contaminated cell cultures. After having discarded the contaminated cells along with the used reagents and cell culture media, we recommend thorough cleaning of the whole laboratory.

However, we appreciate that certain cells and virus stocks are rare or historically significant and might benefit from being cleaned up. With this purpose in mind Minerva Biolabs have developed mycoplasma elimination products (Mynox® and Mynox® Gold) that can eradicate mycoplasma from an already contaminated cell culture with negligible cytotoxicity.

Mynox® is an antibiotic-free formulation for mycoplasma eradication from cell cultures. Its activity is based on a biophysical mechanism and therefore does not allow for the development of resistant strains.


Mynox® Gold is a formulation of the classic Mynox® reagent that has undergone further development and combines a standard antibiotic with the Mynox® ingredients. Achieve 100% eradication of mycoplasma with no cytotoxicity and a low resistance risk.

WaterShield™

Water Disinfection Additive, 200x solution

Minerva Biolabs

Catalogue No.DescriptionPack SizePriceQty
15-3005Water Disinfection Additive, 200x solution10ml sample - one per lab £5.00 Quantity Add to Order
15-3015Water Disinfection Additive, 200x solution15 x 10ml £95.00 Quantity Add to Order
15-3020Water Disinfection Additive, 200x solution3 x 50ml £87.00 Quantity Add to Order
15-3050Water Disinfection Additive, 200x solution500ml £286.00 Quantity Add to Order

Description

Water required for CO2 incubators is prone to microbial contamination due to ideal environmental conditions. WaterShield™ protects the water in the reservoir from microbial growth.


Broad Applicability

WaterShield™ can also be used in regular water/heating baths and is compatible with common work surfaces (e.g. tin plated iron, chrome, nickel steel, high grade steel and copper).


No cell toxicity
The water additive contains quaternary ammonium compounds and surfactants as disinfecting and cleaning ingredients, but no aldehydes, phenols, alcohols or azides. These reagents are not vaporizing and are therefore safe for tissue culture cells. All ingredients are safe to lab workers and do not cause any irritating effects to the skin when used at the recommended concentration. There are no nasty odours.


Broad inactivation range
WaterShield™ is active against most bacteria, mycoplasma, protozoon, algae, fungi and yeast.


Easy to use
Simply add one bottle (5 ml) of WaterShield™ directly to one liter of deionized water.  The easily applicable 200X aqueous solution is provided in aliquots of 5 ml ready-to-go for 1 liter of water.


Stability
The reagent is usually active for 4 weeks. A blue stain indicated the status of the additive. In case of increasing bioburden a renewal of the water and the additive is required

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

Manual and SDS can be found on the manufacturer's website here

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

WaterShield™ can also be used in regular water/heating baths and is compatible with common work surfaces (e.g. tin plated iron, chrome, nickel steel, high grade steel and copper). 

Applicable in research and industry only. Not recommended for clinical applications. Do not use with acrylic glass or aluminum surfaces.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200