5'-Amino-Modifier C12-PDA
atalog Number: 10-1948-xx
Description: 5'-Amino-Modifier C12-PDA
12-(N2-methyl-N1-phthalimidyl)-dodecyl-1-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite |
| Formula: C30H51N4O4P |
M.W.: 562.7 |
F.W.: 263.32 |
Diluent: 25% DCM in Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Synthesize using acetyl-protected dC (10-1015-xx) and deprotect in 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 minutes. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
Our more recent 5’-amino modifiers, protected by a novel phthalic acid diamide (PDA) protecting group, are stable solids . In contrast to the TFA protected amino modifiers, which are viscous oils, the analogous PDA protected compounds are granular powders. This important property of these compounds allows straightforward handling, storage and aliquoting and leads to a significant increase in stability.
Deprotection with methylamine in gas phase or aqueous solution or AMA leads to fast and complete removal of the PDA protecting group. However, ammonium hydroxide will not drive the equilibrium reaction to completion and only partial deprotection occurs - overnight deprotection with ammonium hydroxide will yield around 80% active amine.
We are offering three PDA Amino-Modifiers:
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5’-Amino-Modifier C6-PDA
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Hydrophobic 5’-Amino-Modifier C12-PDA
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Hydrophilic 5’-Amino-Modifier-TEG-PDA
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200