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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

Stearyl Phosphoramidite

Stearyl Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1979-02Stearyl Phosphoramidite0.25g £164.00 Quantity Add to Order
10-1979-90Stearyl Phosphoramidite100 µmole £41.00 Quantity Add to Order

Description

Stearyl Phosphoramidite

Structure

Catalog Number: 10-1979-xx

Description: Stearyl Phosphoramidite

Octadecyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C27H55N2O2P M.W.: 470.71 F.W.: 332.46
Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/ProductFiles/Technical/TB_ABITransfer.pdf.
Coupling: 3 minute coupling time recommended.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

STEARYL LABELLING

We now offer a simple C18 lipid as an economical and effective carrier molecule. We envisage that the 5’-stearyl group will become a favored lipophilic carrier for experimentation with synthetic oligonucleotides.

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Protocols

Material Safety Data Sheet

Glen Report 24.1: New Product - 5'-Stearyl Phosphoramidite - An Alternative Lipophilic Carrier

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1979-90 100µmoles .047grams 1 20 12 7.5 5.45 4 1
10-1979-02 0.25grams .25grams 5.31 163.67 98.2 61.38 44.64 32.73 8.18
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1979-90 100µmoles .047grams 1.5 .07 23.6 14.75 10.73 1.48  
10-1979-02 0.25grams .25grams 7.93 .07 152.2 95.13 69.18 9.51  
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1979-90 100µmoles .047grams 1.5 .07 25.2 15.75 11.45    
10-1979-02 0.25grams .25grams 7.93 .07 153.8 96.13 69.91  

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200