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Water Contamination Testing

Water Contamination Testing: Water Pathogen Testing Kits

A range of kits for extraction and detection of water borne pathogens

AquaScreen® PCR Detection kits - detection of Escherichia coli

The AquaScreen® Escherichia coli qPCR Detection Kit is used for DNA samples prepared with the AquaScreen® FastExtract procedure for quantification of Escherichia coli in water samples.

Minerva Biolabs

Catalogue No.DescriptionPack SizePriceQty
34-7025AquaScreen® Escherichia coli25 rxns £280.00 Quantity Add to Order
34-7100AquaScreen® Escherichia coli100 rxns £1,011.00 Quantity Add to Order
34-7250AquaScreen® Escherichia coli250 rxns £2,246.00 Quantity Add to Order

Description

AquaScreen® Escherichia coli  

Type of PCR

Quantitative real-time PCR (qPCR)

The AquaScreen® Escherichia coli qPCR Detection Kit is used for DNA samples prepared with the AquaScreen® FastExtract procedure for quantification of Escherichia coli in water samples.

Recommended Use / Scope

Applicable in research and industry for QA testing of household and process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

Kit Components

Freeze-dried primer, probes, nucleotides and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control

 

AquaScreen flyer

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Protocols

 

Instructions for use

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References

Felföldi T. et al., (2010). Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary. Clinical Microbiology and Infection, 16: 89–92 10.1111/j.1469-0691.2009.02795.x.

Kirschner A.K. (2016). Determination of viable legionellae in engineered water systems: Do we find what we are looking for?

Water Research, 93:276-88. doi: 10.1016/j.watres.2016.02.016.

Rozwadowska B. et al., (2013). Application of a real-time PCR method for Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens detection in water samples. Polish Journal of Microbiology, 62(4):439-43.

Vogel J. et al., (2013). Impacts of Migratory Sandhill Cranes (Grus canadensis) on Microbial Water Quality in the Central Platte River, Nebraska, USA. Water, Air and Soil Pollution, 224:1576. doi: 10.1007/s11270-013-1576-3.

Yaradou D.F. et al., (2007). Integrated real-time PCR for detection and monitoring of Legionella pneumophila in water systems. Applied and Environmental Microbiology, 73(5):1452-6. doi: 10.1128/AEM.02399-06.

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Notes

Required Consumables

PCR reaction tubes

Optional: For calibration we recommend our Escherichia coli DNA Calibration
Reagent (Cat.-No. 52-0083).

Required lab devices

Pipetting equipment

qPCR cycler with filter sets for FAM™ and ROX™

Shelf Life and Storage

Components are maintainable at 2 to 8 °C for at least 6 months. After rehydratisation the reagents must be stored at -18 °C.

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Applications & Benefits

Recommended Use / Scope

Applicable in research and industry for QA testing of household and process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

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