Transfection-EZ Biosystems: Primary Cells
Avalanche®- primary cell type-specific Transfection Reagent series?
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
If you work on cell lines rather than primary cells then click HERE
Human Cardiac Fibroblasts (HCF, NHCF-A, NHCF-V, AoAF) Avalanche™ Transfection Reagent
Transfection of primary cells, such as Human Cardiac Fibroblasts or Cardiac Related Fibroblasts, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.
Human Cardiac Fibroblasts (HCF, NHCF-A, NHCF-V, AoAF) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Human Cardiac fibroblasts and Cardiac Related Fibroblasts. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Cardiac fibroblasts and Cardiac Related Fibroblasts. No other transfection reagents can match the efficiency, convenience, and gentleness of The Reagent for transfection on Human Cardiac fibroblasts and Cardiac Related Fibroblasts
EZ Biosystems
Description
|
Cell Information:
Human Cardiac Fibroblasts (HCF, NHCF-A and -V) are derived from normal human heart tissues (atrium or ventricle). Aortic Adventitial Fibroblasts (AoAF) (isolated from the tunica external of the ascending or descending aorta) are Cardiac Related fibroblasts . Human Cardiac fibroblasts or Cardiac Related Fibroblasts have been used to study the effects of TNF-alpha and angiotensin (Ang) II levels on fibroblast function. Expression of MyoD can induce myogenesis of cardiac fibroblasts. Cardiac fibroblasts involved in aortic regurgitation synthesize more Fibronectin. Estradiol inhibits cardiac fibroblast growth through conversion to methoxyestradiols, which may protect against remodeling involved in heart disease. TGF-beta stimulates differentiation of fibroblasts to myofibroblasts, which increases collagen production.
Applications:
- Vascular diseases
- Neointimal formation
- Vascular remodeling
- Atherosclerosis
- Hypertension
- Myocardial repair
- Extracellular maintenance during cardiac cell / tissue growth
- Cardiac Fibrosis Paracrine signalling
- Toxicology
- Gene expression
The Transfection Reagent:
Transfection of primary cells, such as Human Cardiac Fibroblasts or Cardiac Related Fibroblasts, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.
Human Cardiac Fibroblasts (HCF, NHCF-A, NHCF-V, AoAF) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Human Cardiac fibroblasts and Cardiac Related Fibroblasts. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Cardiac fibroblasts and Cardiac Related Fibroblasts. No other transfection reagents can match the efficiency, convenience, and gentleness of The Reagent for transfection on Human Cardiac fibroblasts and Cardiac Related Fibroblasts. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in Human Cardiac fibroblasts and Cardiac Related Fibroblasts.
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Compatible with serum
- Suitable for Reverse Transfection
- Developed and manufactured by EZ Biosystems
|
| Data: |
 |
| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human dermal fibroblasts after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human dermal fibroblasts. This test result was confirmed with repeat experiments. The candidate formulas were also tested in human cardiac fibroblasts by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Human Cardiac Fibroblast Avalanche Transfection Reagent. |
| |
 |
| FIG. 2. Human Cardiac Fibroblasts were transfected with GFP vector (pEGFP-N3) by using Human Cardiac Fibroblast Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection. |
| |
|
For Other Cells: |
Human Cardiac Fibroblasts (HCF, NHCF-A, NHCF-V, AoAF) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.
Human Dermal Fibroblast (HDF)
Human Lung Fibroblast (HLF, NHLF)
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200