Transfection-EZ Biosystems: Primary Cells
Avalanche®- primary cell type-specific Transfection Reagent series?
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
If you work on cell lines rather than primary cells then click HERE
Human Epidermal Melanocyte (HEM, NHEM) Avalanche™ Transfection Reagent
Transfection of primary cells, such as HEM, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.
Human Epidermal Melanocyte (HEM, NHEM) Avalanche™ TRansfection Reagent is a new, proprietary solution specifically designed for transfection on HEM. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HEM.
EZ Biosystems
Description
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ell Information:
Human Epidermal Melanocytes (HEM) are the pigment producing cells located in the epidermal layers of all mammalian skin. The function of the melanocyte is to produce melanin, the pigment that gives skin its color. Melanin protects skin from UV damage.
Melanocytes in cosmetic studies are used to test:
- Skin lightening compounds for moles-freckles-nevi-age spots
- Skin darkening products - tan enhancing products
- UV protection compounds - sunscreen
Melanocyte in medical research often centers on melanoma as well as various dermatological diseases or related disorders like:
- Albinism and vitiligo
- Hormonal treatments
- Thyroid conditions
- Psoriasis
- Burns
- Scars
The Transfection Reagent:
Transfection of primary cells, such as HEM, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.
Human Epidermal Melanocyte (HEM, NHEM) Avalanche™ TRansfection Reagent is a new, proprietary solution specifically designed for transfection on HEM. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HEM. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver plasmid DNA into HEM
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Simplest and straightforward protocols
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human epidermal melanocytes after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human epidermal melanocytes. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Epidermal Melanocyte Avalanche Transfection Reagent. |
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For Other Cells: |
Human Epidermal Melanocyte (HEM, NHEM) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200