- All-in-one kit to produce uridine-containing, fully 5’ capped and 3’ poly(A) tailed mRNA (U-mRNA).
- Combine the benefits of high-yield transcription with post-transcriptional capping and tailing.
- Obtain virtually 100% 5’-capped transcripts.
- Create variable 3’ poly(A) tail lengths, from 50 to >300 A’s.
- Two kit sizes to produce 0.6 or 1.5 mg of U-mRNA.
- ScriptGuard™ RNase Inhibitor included to protect synthesized mRNA from RNase degradation.
Product is for research use only (RUO)
Product Description:
The T7 mScript™ Standard mRNA Production System V2 provides all
enzymes and reagents for making canonical uridine (U)-containing,
5'-capped and 3'-polyadenylated mRNA. The kit includes reagents for four workflow modules, (1) high-yield in vitro transcription
of linear double-stranded DNA templates using the T7 mScript™ Enzyme
Solution and the canonical nucleotides ATP, CTP, GTP and UTP, (2)
enzymatic capping of the RNA using the ScriptCap™ Cap 1 Capping System (contains both ScriptCap™ Capping Enzyme and 2'-O-Methyltransferase) for making mRNA with a Cap 1 cap structure, (3) A-Plus™ Poly(A) Polymerase for adding a 3' poly(A) tail and (4) 5 M NH4OAc as a convenient RNA/mRNA purification method. ScriptGuard™ RNase Inhibitor is included in the kit to protect synthesized mRNA from RNase degradation.
Post-transfection, capped and tailed mRNA has increased stability and
translation efficiency in most eukaryotic cell lines. The mScript™
System improves upon co-transcriptional capping and tailing methods by
ensuring virtually 100% transcript capping and enabling users to define
their desired tail lengths, even greater than 300 A's. In this way,
poly(A) tail lengths can be generated much longer than is possible using
a template-encoded tail. mRNAs with longer poly(A) tails are expressed
for longer periods of time in cells. mScript™ U-mRNA is suitable for use
in transfection and microinjection experiments as well as in vitro translation systems.
For reduction of immunogenicity, combine mScript™ U-mRNA with the Min-Immune™ Gold dsRNA Removal Kit
to produce virtually dsRNA-free (to <0.005% of sample [LLOQ]),
ultra-low immunogenicity mRNA that is suitable for downstream
applications such as cell and gene therapy research and mRNA vaccine
development.
CELLSCRIPT™ also offers the INCOGNITO™ T7 mScript™ N1meΨ-mRNA Production System and INCOGNITO™ T7 mScript™ Ψ-mRNA Production System to produce low immunogenicity mRNA containing the modified nucleotides N1-methyl-pseudouridine and pseudouridine, respectively.
Materials Supplied:
Important Store at -20°C in a freezer without a defrost cycle. Do not store at -70°C.
T7 Control Template DNA: Is a linearized 4.1 kb
plasmid that contains a T7 promoter followed by a phage lambda dsDNA
insert that encodes a 1,375 base runoff transcript. The Control Template
DNA is provided at a concentration of 0.5 µg/µl in T10E1 Buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA).
Materials Required, but not Supplied
- A DNA template for transcription of your RNA of interest
- Materials or kits for purification of the RNA product
- RNase-free TE Buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA)
- Optional: TE saturated phenol/chloroform, 0.5-1 M EDTA
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200